Detailed information of TA system TA235825

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69768..70021
• Replicon: plasmid pISHS7_2
• Accession: NZ_CP092254
• Organism: Escherichia coli strain ISHS7

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MF614_RS24215
• Protein ID: WP_001312851.1
• Coordinates: 69872..70021 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69768..69827 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF614_RS24190 (66495)	66495..67241	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MF614_RS24195 (67296)	67296..67856	+	561	WP_000139328.1	fertility inhibition protein FinO	-
MF614_RS24200 (67988)	67988..68188	+	201	WP_015059022.1	hypothetical protein	-
MF614_RS24205 (68574)	68574..69173	+	600	WP_032083981.1	PIN domain-containing protein	-
MF614_RS24210 (69235)	69235..69567	+	333	WP_152916585.1	hypothetical protein	-
- (69768)	69768..69827	-	60	NuclAT_1	-	Antitoxin
- (69768)	69768..69827	-	60	NuclAT_1	-	Antitoxin
- (69768)	69768..69827	-	60	NuclAT_1	-	Antitoxin
- (69768)	69768..69827	-	60	NuclAT_1	-	Antitoxin
MF614_RS24215 (69872)	69872..70021	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MF614_RS24220 (70305)	70305..70553	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-55 / rmtB	-	1..70864	70864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T235825 WP_001312851.1 NZ_CP092254:69872-70021 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T235825 NZ_LR134383:c558141-557821 [Legionella jordanis]
ATGAATCGTGGTGATGTATATTGGGTAAACCTTGACCCTACTACTGGATCTGAGATTAATAAACTCAGACCTTGTGTTTT
AGTTGGAGCAACACCAATTAATCAAGCAAGACGCACTGTTGTTGTCGTTCCCCTTTCAACTTCTGCCACAGCCAGGCCGC
CAATAACTATCTCTGTTTCCTGTCTTGGTAAACAAGTGACAGCTGTTTGTGATCAAATAAGAACTGTCGATAAAAGTCGA
TTAAAAAACGTTGCAGGGAGCCTTTCAGATAAAGACTTAAATGCTTTGGATGACGGACTGAGACAAATTTTATGCCTATA
A

Antitoxin

Nucleotide

• Length: 60 bp

>AT235825 NZ_CP092254:c69827-69768 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin