Detailed information of TA system TA235822

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7668..8094
• Replicon: plasmid pISHS7_2
• Accession: NZ_CP092254
• Organism: Escherichia coli strain ISHS7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MF614_RS23825
• Protein ID: WP_001372321.1
• Coordinates: 7668..7793 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	7870..8094 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF614_RS23790 (2733)	2733..3134	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MF614_RS23795 (3419)	3419..4729	+	1311	WP_001262765.1	DUF262 domain-containing protein	-
MF614_RS23800 (4758)	4758..4856	+	99	Protein_5	IS1 family transposase	-
MF614_RS23805 (5006)	5006..5749	-	744	Protein_6	class A beta-lactamase	-
MF614_RS23810 (5799)	5799..6503	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MF614_RS23815 (6550)	6550..6753	-	204	WP_239371673.1	hypothetical protein	-
MF614_RS23820 (7053)	7053..7349	+	297	Protein_9	hypothetical protein	-
MF614_RS23825 (7668)	7668..7793	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MF614_RS23830 (7735)	7735..7884	-	150	Protein_11	plasmid maintenance protein Mok	-
MF614_RS23835 (7906)	7906..8085	+	180	WP_001309233.1	hypothetical protein	-
- (7870)	7870..8094	-	225	NuclAT_0	-	Antitoxin
- (7870)	7870..8094	-	225	NuclAT_0	-	Antitoxin
- (7870)	7870..8094	-	225	NuclAT_0	-	Antitoxin
- (7870)	7870..8094	-	225	NuclAT_0	-	Antitoxin
MF614_RS23840 (8063)	8063..8825	-	763	Protein_13	plasmid SOS inhibition protein A	-
MF614_RS23845 (8822)	8822..9256	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MF614_RS23850 (9325)	9325..11348	-	2024	Protein_15	ParB/RepB/Spo0J family partition protein	-
MF614_RS23855 (11409)	11409..11642	-	234	WP_000006003.1	DUF905 family protein	-
MF614_RS23860 (11700)	11700..12227	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
MF614_RS23865 (12529)	12529..13008	+	480	Protein_18	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaCTX-M-55 / rmtB	-	1..70864	70864
	-	flank	IS/Tn	blaTEM-1B	-	5006..6503	1497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T235822 WP_001372321.1 NZ_CP092254:c7793-7668 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T235822 NZ_LR134379:1186705-1186986 [Slackia heliotrinireducens]
ATGTCCTTGAAGCTCGTTCCCACATCGCAGTTCAAGAAGGATTATAAGAGGGCGAAGAAGCGTGGGTTGGACATGCGCAA
GCTCCAGGCTGTGCTTGACAAGCTTTGTGCCGAGGAGCCGCTCGACGAACGCCATCGCGACCATGCGCTATCGGGCAACT
ACATTGGATTTCGTGAATGCCATGTTAGCCCCGATTGGCTGCTGATATACGCGATTGACAAGGGCCGTCTGATACTTACC
GCATCGCGAACAGGGACGCACTCGGATTTGTTCGACGAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT235822 NZ_CP092254:c8094-7870 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin