Detailed information of TA system TA235818

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40670..40934
• Replicon: plasmid pISHS7_1
• Accession: NZ_CP092253
• Organism: Escherichia coli strain ISHS7

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: MF614_RS23535
• Protein ID: WP_042078455.1
• Coordinates: 40782..40934 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	40670..40727 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF614_RS23520 (35909)	35909..38200	-	2292	WP_077134482.1	F-type conjugative transfer protein TrbC	-
MF614_RS23525 (38193)	38193..39263	-	1071	WP_239371658.1	IncI1-type conjugal transfer protein TrbB	-
MF614_RS23530 (39282)	39282..40490	-	1209	WP_077134481.1	IncI1-type conjugal transfer protein TrbA	-
- (40670)	40670..40727	-	58	NuclAT_0	-	Antitoxin
- (40670)	40670..40727	-	58	NuclAT_0	-	Antitoxin
- (40670)	40670..40727	-	58	NuclAT_0	-	Antitoxin
- (40670)	40670..40727	-	58	NuclAT_0	-	Antitoxin
MF614_RS23535 (40782)	40782..40934	+	153	WP_042078455.1	Hok/Gef family protein	Toxin
MF614_RS23540 (41006)	41006..41257	-	252	WP_023225283.1	hypothetical protein	-
MF614_RS24580 (41756)	41756..41851	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
MF614_RS23550 (41916)	41916..42092	-	177	WP_001054898.1	hypothetical protein	-
MF614_RS23555 (42424)	42424..42633	+	210	WP_239371660.1	HEAT repeat domain-containing protein	-
MF614_RS23560 (42705)	42705..43367	-	663	WP_042078443.1	plasmid IncI1-type surface exclusion protein ExcA	-
MF614_RS23565 (43437)	43437..45605	-	2169	WP_042078442.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..84986	84986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5789.15 Da
• Isoelectric Point: 8.7948

>T235818 WP_042078455.1 NZ_CP092253:40782-40934 [Escherichia coli]
MPQRTFLMMLIVICVTILCFIWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T235818 NZ_LR134378:c1794051-1793761 [Lautropia mirabilis]
ATGCGTCAGCGCTTCACTTGGGATCCAGCCAAAGCCGCCAGCAACCTGCGCAAGCATGGCATTTCCTTTGAGGAGGCCGT
TTGCGTCTTCGCCGATCCAATGGCCGTTACACGCCAGGATCGCATCGAGAACGGCGAGCAACGCTGGCAGACCATTGGCG
CAGTCGGTGGCTTTACCTTGCTTCTGGTGGCCCACACGCTGTGGGATGACGAAGCAGGCATTGAGGTCGTGCGCATCATC
AGTGCTCGTCGTGCAGACCGAAAGGAACGGAGAATCTATGAAAACGGTTAG

Antitoxin

Nucleotide

• Length: 58 bp

>AT235818 NZ_CP092253:c40727-40670 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin