Detailed information of TA system TA235817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4639086..4639688
• Replicon: chromosome
• Accession: NZ_CP092252
• Organism: Escherichia coli strain ISHS7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MF614_RS22325
• Protein ID: WP_000897305.1
• Coordinates: 4639377..4639688 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MF614_RS22320
• Protein ID: WP_000356397.1
• Coordinates: 4639086..4639376 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF614_RS22295 (4635012)	4635012..4635914	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MF614_RS22300 (4635911)	4635911..4636546	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MF614_RS22305 (4636543)	4636543..4637472	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
MF614_RS22310 (4637802)	4637802..4638044	-	243	WP_001087409.1	protein YiiF	-
MF614_RS22315 (4638263)	4638263..4638481	-	219	WP_001295676.1	CopG family transcriptional regulator	-
MF614_RS22320 (4639086)	4639086..4639376	-	291	WP_000356397.1	NadS family protein	Antitoxin
MF614_RS22325 (4639377)	4639377..4639688	-	312	WP_000897305.1	hypothetical protein	Toxin
MF614_RS22330 (4639917)	4639917..4640825	+	909	WP_001162704.1	alpha/beta hydrolase	-
MF614_RS22335 (4640889)	4640889..4641830	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MF614_RS22340 (4641875)	4641875..4642312	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MF614_RS22345 (4642309)	4642309..4643181	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MF614_RS22350 (4643175)	4643175..4643774	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T235817 WP_000897305.1 NZ_CP092252:c4639688-4639377 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T235817 NZ_LR134378:c1171717-1171433 [Lautropia mirabilis]
ATGAAGTATCAGCTCACCTTCAACCCCCAAGCCCTGAAGGAGTTCAGGAAACTGGGGGCAAACATCGCCAATCAGTTTCT
CGACAAACTTGAGGAGCGACTGCAAAACCCGAAGGTGCCCAGCGCCCGCCTTATCGGCATGGCCGACTGCTACAAGATCA
AGCTTCGCAGCAGTGGCTATCGTCTGGTCTATCAGGTACAAGACGAGAAGATCATCGTCCAAGTCATCGCCGTGGGAAAG
CGTGAGCGTATGGAGGTCTATCAGAAAGCGGCACGACGGCTGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT235817 WP_000356397.1 NZ_CP092252:c4639376-4639086 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT235817 NZ_LR134378:c1171968-1171714 [Lautropia mirabilis]
ATGCCCAGCCTGATCCTGACCGATACCGTCGCCAGCCTTGCCGAGCTGAAAGAGGCCCCTCTGGAAACTTTCAGCGCTGC
TGATGGACACGCCATCGCTATCCTGGACAAGGACAAACCAGCCTTCTATTGCGTATCGCCCGAACTCTATGAGTACTTCA
TGGAGCTGGCCGAAGATGCCGAGCTGACCAGAATCGTGGAAGAGCGCCAGCACGAGGAGCCCATTCCCGTGGAGCTGGAG
TCGCTGCGTCGATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7