Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629737..630536 Replicon chromosome
Accession NZ_CP092252
Organism Escherichia coli strain ISHS7

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag MF614_RS03120 Protein ID WP_000347267.1
Coordinates 629737..630201 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MF614_RS03125 Protein ID WP_001307405.1
Coordinates 630201..630536 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF614_RS03090 (624738) 624738..625172 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MF614_RS03095 (625190) 625190..626068 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
MF614_RS03100 (626058) 626058..626837 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MF614_RS03105 (626848) 626848..627321 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MF614_RS03110 (627344) 627344..628624 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
MF614_RS03115 (628873) 628873..629682 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MF614_RS03120 (629737) 629737..630201 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MF614_RS03125 (630201) 630201..630536 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MF614_RS03130 (630685) 630685..632256 - 1572 WP_001273741.1 galactarate dehydratase -
MF614_RS03135 (632631) 632631..633965 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MF614_RS03140 (633981) 633981..634751 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 629737..642471 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T235798 WP_000347267.1 NZ_CP092252:c630201-629737 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T235798 NZ_LR134360:991217-991423 [Staphylococcus condimenti]
ATGCAAAACAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTACATCAACATCTTATAGAAAT
GACAGGTGTAACTGCAGTACAAGTAGATTTGGATTCAAATGAAATCAAAATCAGTTACGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCGGGTTATCAAATATTAAATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT235798 WP_001307405.1 NZ_CP092252:c630536-630201 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT235798 NZ_LR134360:990912-991205 [Staphylococcus condimenti]
ATGACCGAAAATGACAAAGCACATCATTCAGAACAAATTAAGTCCAACTTGAAAGCAAGACTCAACCGTATTGAGGGGCA
AGTCAGAGCAATTAACCGAATGATTGAAGAAGATGTTTATTGTGATGATGTACTTACACAAATCCGTGCAACACGTTCAG
CCTTGAACAGTGTAGCAGTACGATTGCTTGATCACCATATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCAATGGAAGAATTGCTTGTTACCGTTCAAAAATTAATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References