Detailed information of TA system TA235758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 242699..243289
• Replicon: chromosome
• Accession: NZ_CP092182
• Organism: Alcaligenes faecalis strain T17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A2N9E1
• Locus tag: MF263_RS01090
• Protein ID: WP_035268575.1
• Coordinates: 242993..243289 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: MF263_RS01085
• Protein ID: WP_042484646.1
• Coordinates: 242699..242986 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MF263_RS01070 (MF263_01070)	238486..239733	+	1248	WP_083055250.1	D-amino acid dehydrogenase	-
MF263_RS01075 (MF263_01075)	239932..240942	-	1011	WP_042484652.1	acyltransferase family protein	-
MF263_RS01080 (MF263_01080)	241221..242588	+	1368	WP_239054989.1	DNA recombination protein RmuC	-
MF263_RS01085 (MF263_01085)	242699..242986	-	288	WP_042484646.1	HigA family addiction module antitoxin	Antitoxin
MF263_RS01090 (MF263_01090)	242993..243289	-	297	WP_035268575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MF263_RS01095 (MF263_01095)	243424..245973	-	2550	WP_239054990.1	EAL domain-containing protein	-
MF263_RS01100 (MF263_01100)	246166..246444	+	279	WP_009460160.1	acylphosphatase	-
MF263_RS01105 (MF263_01105)	246609..247502	+	894	WP_239054991.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11108.48 Da
• Isoelectric Point: 9.3459

>T235758 WP_035268575.1 NZ_CP092182:c243289-242993 [Alcaligenes faecalis]
MIKSFRHKGLQAYFETGSKSGIQAKHAVRLQIQLTALHSATQAEDMNAPGWKLHKLSGKNPKGQSVQDHYTISVSGNWRL
TFYFEGEDAVLVDYQDYH

Nucleotide

• Length: 297 bp

>T235758 NZ_LR134348:c967468-967145 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10430.96 Da
• Isoelectric Point: 9.8929

>AT235758 WP_042484646.1 NZ_CP092182:c242986-242699 [Alcaligenes faecalis]
IMRMHNPSHPGPVLREYLGELSVTEAASRLGVTRAALSRILNGSAGISADMALRLRDALGTSAEMWINMQSHYDLWQAEQ
KPRPKVVSFHGPAHA

Nucleotide

• Length: 288 bp

>AT235758 NZ_LR134348:c967700-967455 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A2N9E1