Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 68611..69338 | Replicon | plasmid pP1-1 |
| Accession | NZ_CP092078 | ||
| Organism | Klebsiella pneumoniae strain P1-1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | MDE60_RS25515 | Protein ID | WP_011251285.1 |
| Coordinates | 68611..68922 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | MDE60_RS25520 | Protein ID | WP_011251286.1 |
| Coordinates | 68919..69338 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MDE60_RS25485 (MDE60_25460) | 63802..64782 | + | 981 | WP_012569499.1 | IS5-like element ISKpn26 family transposase | - |
| MDE60_RS25490 (MDE60_25465) | 65328..65507 | - | 180 | Protein_70 | IS5/IS1182 family transposase | - |
| MDE60_RS25495 (MDE60_25470) | 65547..67085 | - | 1539 | WP_017901237.1 | IS66 family transposase | - |
| MDE60_RS25500 (MDE60_25475) | 67134..67481 | - | 348 | WP_032430752.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| MDE60_RS25505 (MDE60_25480) | 67478..67855 | - | 378 | WP_004118218.1 | transposase | - |
| MDE60_RS25510 (MDE60_25485) | 67969..68406 | + | 438 | Protein_74 | DDE-type integrase/transposase/recombinase | - |
| MDE60_RS25515 (MDE60_25490) | 68611..68922 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| MDE60_RS25520 (MDE60_25495) | 68919..69338 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| MDE60_RS25525 (MDE60_25500) | 69408..69821 | + | 414 | WP_017901337.1 | hypothetical protein | - |
| MDE60_RS25530 (MDE60_25505) | 69867..70835 | - | 969 | WP_077253741.1 | IS5-like element IS903B family transposase | - |
| MDE60_RS25535 (MDE60_25510) | 70939..71205 | + | 267 | WP_017900868.1 | hypothetical protein | - |
| MDE60_RS25540 (MDE60_25515) | 71319..71854 | - | 536 | Protein_80 | transposase | - |
| MDE60_RS25545 (MDE60_25520) | 72379..73746 | - | 1368 | WP_017900870.1 | formimidoylglutamate deiminase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(D) / blaCTX-M-14 / blaDHA-1 / qnrB4 | - | 1..241435 | 241435 | |
| - | inside | IScluster/Tn | - | - | 63802..71713 | 7911 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T235694 WP_011251285.1 NZ_CP092078:68611-68922 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T235694 NZ_LR134329:c2567811-2567386 [Bordetella parapertussis]
ATGATCCTGCTCGACACCGACGTGCTGCTCGAGCCGCTGCGGCCGGCCCCCGACCCGGCCGTCGCGGCATGGCTGGATGC
CCAGCACGTCGAAACCCTGTACCTGGCCGCGCCCGGCGCGGCCGAAATCCAGCTCGGGCTGGCCCGCCTGCCGCGCGGCA
AGCGGGCCGAGGCCTTGCGCCACGAATTCGAGCGGCGCGTCCTGCCGCTGTTCATGGGCCATGTCCTGCCCTTCGACACG
GCCGCGGCCGATGCCTACGCCGCCGTCATGATGCGGGCCGCGGCGGCGGGCCATGCGCTGTGCGCAATCGATGGTTGCAT
CGCCGCCATCGCCACCGCGCACGGCCTGGCCATCGCCACGCGCCATCCGGCCAGGTTCGAGGCGGCCGGGCTCGCCGCCG
TCGATCCCTGGCACGCCGCCGCTTGA
ATGATCCTGCTCGACACCGACGTGCTGCTCGAGCCGCTGCGGCCGGCCCCCGACCCGGCCGTCGCGGCATGGCTGGATGC
CCAGCACGTCGAAACCCTGTACCTGGCCGCGCCCGGCGCGGCCGAAATCCAGCTCGGGCTGGCCCGCCTGCCGCGCGGCA
AGCGGGCCGAGGCCTTGCGCCACGAATTCGAGCGGCGCGTCCTGCCGCTGTTCATGGGCCATGTCCTGCCCTTCGACACG
GCCGCGGCCGATGCCTACGCCGCCGTCATGATGCGGGCCGCGGCGGCGGGCCATGCGCTGTGCGCAATCGATGGTTGCAT
CGCCGCCATCGCCACCGCGCACGGCCTGGCCATCGCCACGCGCCATCCGGCCAGGTTCGAGGCGGCCGGGCTCGCCGCCG
TCGATCCCTGGCACGCCGCCGCTTGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT235694 WP_011251286.1 NZ_CP092078:68919-69338 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT235694 NZ_LR134329:c2568047-2567808 [Bordetella parapertussis]
GTGTCTTCGATTACGGTAAGAAATGTTCCCGAGGAAGTGCATCGCGCCTTGCGCGTACGCGCGGCCACGCATGGGCGCAG
CACCGAGGCCGAGATCCGCGCCATCCTGGAAAACGCCGTGCTGCCCGATGGCCGCGTCAAGCTGGGCACCCTGCTCAGCC
ACATCGGCCGCGGCGCGCGCGGCATGGACCTGACCATCGAGCGCAATCCGGCGCCCGCTCGCCCGGCCAGCTTCGAATGA
GTGTCTTCGATTACGGTAAGAAATGTTCCCGAGGAAGTGCATCGCGCCTTGCGCGTACGCGCGGCCACGCATGGGCGCAG
CACCGAGGCCGAGATCCGCGCCATCCTGGAAAACGCCGTGCTGCCCGATGGCCGCGTCAAGCTGGGCACCCTGCTCAGCC
ACATCGGCCGCGGCGCGCGCGGCATGGACCTGACCATCGAGCGCAATCCGGCGCCCGCTCGCCCGGCCAGCTTCGAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|