Detailed information of TA system TA023569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1076747..1077366
• Replicon: chromosome
• Accession: NC_012108
• Organism: Desulfobacterium autotrophicum HRM2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0QKI2
• Locus tag: HRM2_RS04620
• Protein ID: WP_015902848.1
• Coordinates: 1077067..1077366 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRM2_RS04615
• Protein ID: WP_015902847.1
• Coordinates: 1076747..1077058 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRM2_RS25855	1072023..1072133	-	111	WP_012663286.1	hypothetical protein	-
HRM2_RS04585	1072240..1072503	-	264	WP_012663287.1	DUF2442 domain-containing protein	-
HRM2_RS04590	1072515..1072775	-	261	WP_041273049.1	DUF4160 domain-containing protein	-
HRM2_RS04595	1073216..1073590	-	375	WP_012663288.1	HTH domain-containing protein	-
HRM2_RS04600	1073587..1073904	-	318	WP_012663289.1	hypothetical protein	-
HRM2_RS04605	1074090..1074404	+	315	WP_015902845.1	hypothetical protein	-
HRM2_RS04610	1074439..1076115	-	1677	WP_015902846.1	IS1634 family transposase	-
HRM2_RS04615	1076747..1077058	-	312	WP_015902847.1	HigA family addiction module antidote protein	Antitoxin
HRM2_RS04620	1077067..1077366	-	300	WP_015902848.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRM2_RS04625	1078068..1078778	+	711	WP_015902849.1	hypothetical protein	-
HRM2_RS04630	1078818..1080173	-	1356	WP_012663192.1	IS4 family transposase	-
HRM2_RS04635	1080333..1081013	+	681	WP_083776516.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11880.64 Da
• Isoelectric Point: 9.9375

>T23569 WP_015902848.1 NC_012108:c1077366-1077067 [Desulfobacterium autotrophicum HRM2]
MITTFRHKWLQSFFIDDVNHKKIPASIRDVLFRKLQVIDDATCDMDLRSPPSNHFEKLTGTLKGKHSIRINKQWRLIFKW
NDIRGEAEHIYLDNHDYKS

Nucleotide

• Length: 300 bp

>T23569 NC_012108:c1077366-1077067 [Desulfobacterium autotrophicum HRM2]
ATGATTACAACGTTTCGACATAAATGGCTTCAGAGCTTTTTTATCGACGACGTAAATCATAAAAAAATACCTGCTTCAAT
CCGAGATGTTCTTTTTAGAAAGCTACAAGTCATTGATGATGCAACATGTGATATGGATTTGCGGAGCCCCCCAAGCAACC
ATTTTGAAAAACTAACCGGGACTTTAAAAGGAAAACATTCCATACGGATTAACAAGCAATGGAGATTAATATTTAAATGG
AACGACATACGGGGCGAAGCTGAACATATTTATCTGGACAACCATGATTACAAATCATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11874.95 Da
• Isoelectric Point: 10.9818

>AT23569 WP_015902847.1 NC_012108:c1077058-1076747 [Desulfobacterium autotrophicum HRM2]
MITTKRKPVSVGEIITEEYLKPLKITQAQLSQAMGVSRKTVNELCTGKRAITVDTALMLARVFENTPEFWLNLQRRIDIW
NVLHNPERKARIEKARPLRNAFA

Nucleotide

• Length: 312 bp

>AT23569 NC_012108:c1077058-1076747 [Desulfobacterium autotrophicum HRM2]
ATGATAACTACAAAAAGAAAACCGGTAAGTGTAGGGGAAATCATAACCGAAGAATATCTTAAACCATTGAAAATCACCCA
GGCACAACTTAGCCAGGCAATGGGGGTAAGCCGCAAAACCGTCAATGAGCTATGTACTGGAAAAAGGGCAATAACCGTTG
ATACCGCACTAATGCTGGCACGAGTATTTGAAAACACACCAGAATTCTGGCTGAACTTACAAAGACGTATCGACATATGG
AACGTCTTACACAATCCGGAACGAAAAGCGAGAATAGAAAAAGCCCGCCCCCTCAGAAACGCCTTTGCCTGA

Structures

Toxin

• AlphaFold DB: C0QKI2