Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3784545..3785142 Replicon chromosome
Accession NZ_CP092077
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MDE60_RS18465 Protein ID WP_004142563.1
Coordinates 3784825..3785142 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MDE60_RS18460 Protein ID WP_004142561.1
Coordinates 3784545..3784832 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS18430 (MDE60_18405) 3780625..3780873 + 249 WP_002893037.1 DUF1158 domain-containing protein -
MDE60_RS18435 (MDE60_18410) 3780891..3781232 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
MDE60_RS18440 (MDE60_18415) 3781263..3782378 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
MDE60_RS18445 (MDE60_18420) 3782558..3783139 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
MDE60_RS18450 (MDE60_18425) 3783139..3783507 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
MDE60_RS18455 (MDE60_18430) 3783627..3784280 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
MDE60_RS18460 (MDE60_18435) 3784545..3784832 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MDE60_RS18465 (MDE60_18440) 3784825..3785142 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MDE60_RS18470 (MDE60_18445) 3785327..3786370 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
MDE60_RS18475 (MDE60_18450) 3787040..3787906 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
MDE60_RS18480 (MDE60_18455) 3788015..3789442 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T235687 WP_004142563.1 NZ_CP092077:c3785142-3784825 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T235687 NZ_LR134326:c2102025-2101450 [Bordetella bronchiseptica]
ATGAGGTACTCCCCCTTCACTGTCATCTTCGATGCCTGTGTTCTCTATCCTGCGCCGCTGCGCGACTTTCTGATGTGGCT
AGGGTTGTCGGGACGGGTCCGTGCTCGCTGGAGTCGCGACATTCATAAGGAGTGGAAACGCAATTTGCTACTCAACAGGC
CTGATCTGACACGTGCACAGGTGGATCGGACTTCAGACCTGATGGATCGCGCAATCCCCGATGGTTTGGTAGATGGGTAT
GAAGATCTCATTGCAGGGTTGACTCTGCCCGACCCCGACGATCGCCATGTTCTCGCTACGGCCATCCGCTGCGGTGCCAG
CGTGATCGTGACCTTCAATCAACGGGATTTCCCAGCAGACGTGCTCGCTTCCTACGGAGTTGAATCTCAACATCCCGACG
AGTTTGTGGAGAATCTCTTTGACCTTGATGCAGCAGCGGTGGTAGCCGCCGCGCAGCGACAGCGGGCGCAACTCAAGAAT
CCGCCTGTTGACGTCGACCATTATCTGGAGATCCTATTCCGGCAAGGACTAGTTCAAACGGTCAAAAACTTAACGACCTA
TCGCTCCATTCTCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT235687 WP_004142561.1 NZ_CP092077:c3784832-3784545 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT235687 NZ_LR134326:c2102489-2102022 [Bordetella bronchiseptica]
ATGACCACCACCACCCCAACCAAGATGACGCTGCCCGCCGAACGAGAGGTCCAGGCAGCCGTGCAGGGGCAACGCGCCTT
GGCGGCCTACTTGGCAACGCGCTTCGAAACACAACACATCCAGATCTTCGATGACCGGAACCAAGCCCATCAGGTGGAGT
TGCCTACCTCCGCGCTGCGTTTGTTGGTCGATATCCTGGCCGAGTTGGCTGGCGGCAATGCCGTAAAAGTCGTACCCGTC
CATGCGGAGCTGACGACCCAGGAAGCTGCGGACATGCTCAACGTCTCCCGCCCTCATCTGGTCAAGCTACTGGAGGATGG
CGTGCTGCCGTTCCATCGAACGGGGAAGCATCGCCGGGTGAGGTTCGCGGACCTGATGCAGTACAAGGATACACGCGACC
GCGCTAGCGAGGAGGCCATGGCCGAACTCGCTCGCCAGGCCCAGGAATCAGGCATGGGGTACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References