Detailed information of TA system TA235676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 353871..354517
• Replicon: chromosome
• Accession: NZ_CP092077
• Organism: Klebsiella pneumoniae strain P1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MDE60_RS01630
• Protein ID: WP_102011763.1
• Coordinates: 353871..354218 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: MDE60_RS01635
• Protein ID: WP_002920557.1
• Coordinates: 354218..354517 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MDE60_RS01620 (MDE60_01620)	349797..351230	+	1434	WP_102011764.1	glycogen synthase GlgA	-
MDE60_RS01625 (MDE60_01625)	351248..353695	+	2448	WP_002920561.1	glycogen phosphorylase	-
MDE60_RS01630 (MDE60_01630)	353871..354218	+	348	WP_102011763.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MDE60_RS01635 (MDE60_01635)	354218..354517	+	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
MDE60_RS01640 (MDE60_01640)	354580..356088	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
MDE60_RS01645 (MDE60_01645)	356293..356622	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
MDE60_RS01650 (MDE60_01650)	356673..357503	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
MDE60_RS01655 (MDE60_01655)	357553..358311	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13535.49 Da
• Isoelectric Point: 5.2054

>T235676 WP_102011763.1 NZ_CP092077:353871-354218 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDNDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T235676 NZ_LR134321:c3553256-3552816 [Shewanella baltica]
GTGGAAGACCGGCACCATAGTTTCAGTGTAAAAGCCTCCTTCGACCAACGACTCTCGTTGGTCGTTTTTATTTGTGTCTG
CTCTAGCTCATTTCTGCTCTGGCCTCAAAGCGACAATCTCACCTTATCCCTGCTTAAATATATGTTTATCCTGCTAGTTT
GTATTTTCTTGCTGTCGCAGTTGTGGCGACTGCAGCATTGGCGTTTAGATTTTGTGCTTAGTGATAAAGGCGAGGGACGT
TTGTCAACGGGTGAGCACTTTCAGGTATTAAGGCGAACTTGGGTGACACCCTTTGTTTGCTTGATGTATATCGAAGTGGA
TACTCAGTTGCGGCTCTTGATGGTTTGGGCGGATATGCTAGACGATACTGACTATCGGCATTTATGTCGGCTGTTGCTGC
GGGCTAAAATACAACAGACTAAACCTCATACCGAGATTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT235676 WP_002920557.1 NZ_CP092077:354218-354517 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT235676 NZ_LR134321:c3553485-3553237 [Shewanella baltica]
TTGGAATTAATGAACATCGCAAGGGTTCGCTGGGCATGTCGCCGCGGGATGCTTGAATTAGACGTTTTGTTTCAACCTTT
TGTTGAAAATTTCTATCAAGAAATGTCCGATCAAGATAAAACCGTGTTTATCCGTTTGTTGGAATGTGAAGATCCCGAAT
TGTTTGCATGGTTTATGGGTCATGAAGTGTGCCCTGATGCTGAGCTTGCGCGCATGGTAGTGAAAGTCCGTGGAAGACCG
GCACCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0E1CBF8