Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 327683..328269 Replicon chromosome
Accession NZ_CP092077
Organism Klebsiella pneumoniae strain P1-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MDE60_RS01520 Protein ID WP_002920800.1
Coordinates 327901..328269 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MDE60_RS01515 Protein ID WP_004174006.1
Coordinates 327683..327904 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDE60_RS01495 (MDE60_01495) 323840..324766 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MDE60_RS01500 (MDE60_01500) 324763..326040 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MDE60_RS01505 (MDE60_01505) 326037..326804 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MDE60_RS01510 (MDE60_01510) 326806..327519 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MDE60_RS01515 (MDE60_01515) 327683..327904 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MDE60_RS01520 (MDE60_01520) 327901..328269 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MDE60_RS01525 (MDE60_01525) 328542..329858 + 1317 WP_032447920.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MDE60_RS01530 (MDE60_01530) 329965..330852 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MDE60_RS01535 (MDE60_01535) 330849..331694 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MDE60_RS01540 (MDE60_01540) 331696..332766 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 324763..333503 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T235675 WP_002920800.1 NZ_CP092077:327901-328269 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T235675 NZ_LR134321:2778914-2779207 [Shewanella baltica]
ATGCCACAAATAATCTTCACTGCCACAGCATTACGTGATTTAGAGCGCTTACGTGAGTTTCTTCGCAGTAAAAATCCTCC
CGCCGCACAACGGGCTGCCAGCGCCATAATCAATACCATTAGAAAACTGGAAAGCTATCCAGACATTGGCCGGCCTATCG
ATGACAATGACTTCAATTTTAGGGAACTGCTGATTGACTTTGGTGACACGGGTTATTTGGCAATGTACCAGTATGATGGC
GGCGAGCGATTGACCGTATTGTGTGTTCGACATCAAAAGGAAGCTGGCTATTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT235675 WP_004174006.1 NZ_CP092077:327683-327904 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT235675 NZ_LR134321:2778642-2778926 [Shewanella baltica]
ATGAGCACAATCAAACCCGTGTCGGTAAAATTAGATGCCGATATTAAAGCCAGAGTCGAGCATTTAGCCGAAACCCGTAA
ACGTTCATCACACTGGATGATGCGTGAAGCGATCCGTGAATACGTTGAGAGAGAAGAGAAACGCGAGGCGTTGCAGCAAG
AAGCGTTACGCGCATGGGAAGAACACCAGACATCAGGCTTGCATGTCACCGGTGACGAAGTGGTGAGTTGGCTGGAGTCC
TGGGGAAGTGAAAATGAACAGGCAGCCCCTACATGCCACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References