Detailed information of TA system TA235673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4522307..4523061
• Replicon: chromosome
• Accession: NZ_CP092075
• Organism: Salmonella enterica strain P281972

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: MC377_RS22200
• Protein ID: WP_000558166.1
• Coordinates: 4522307..4522618 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MC377_RS22205
• Protein ID: WP_001259011.1
• Coordinates: 4522615..4523061 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC377_RS22170 (4517973)	4517973..4518875	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
MC377_RS22175 (4518872)	4518872..4519507	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MC377_RS22180 (4519504)	4519504..4520433	+	930	WP_000027734.1	formate dehydrogenase accessory protein FdhE	-
MC377_RS22185 (4520471)	4520471..4520845	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	-
MC377_RS22190 (4520845)	4520845..4521087	-	243	WP_001523745.1	CopG family transcriptional regulator	-
MC377_RS22195 (4521292)	4521292..4522221	+	930	WP_058661473.1	alpha/beta hydrolase	-
MC377_RS22200 (4522307)	4522307..4522618	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
MC377_RS22205 (4522615)	4522615..4523061	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
MC377_RS22210 (4523076)	4523076..4524017	-	942	WP_238727967.1	fatty acid biosynthesis protein FabY	-
MC377_RS22215 (4524062)	4524062..4524499	-	438	WP_000560956.1	D-aminoacyl-tRNA deacylase	-
MC377_RS22220 (4524496)	4524496..4525368	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MC377_RS22225 (4525362)	4525362..4525961	-	600	WP_000965695.1	glucose-1-phosphatase	-
MC377_RS22230 (4526152)	4526152..4526955	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
MC377_RS22235 (4526989)	4526989..4527885	-	897	WP_001637558.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T235673 WP_000558166.1 NZ_CP092075:4522307-4522618 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T235673 NZ_LR134321:c1666211-1665957 [Shewanella baltica]
ATGAACAAAAAACATTTAAGAACGTTGACGGCTATCTTTGCTAGGCCTGTCAGCGGAGCTATCAAGTGGTCTGATATTGA
AGCGCTGTTTATTGCTCTAGGGGCTGACATTGAAGAGCGTGAGGGGTCTCGAATTGGTGTGGTGCTGTTTGGTGAAGTTC
AGGTATATCACCGCCCACATCCTCAGAAAGAAACTGACAAAGACGCAGTGATAAGCATCAAAAAATGGTTAGAAAGGAAC
GGGGTGAAAGCATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT235673 WP_001259011.1 NZ_CP092075:4522615-4523061 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT235673 NZ_LR134321:c1665960-1665619 [Shewanella baltica]
ATGAAAAATCTTATGATGATCAACGGGGTAAAAGCCTTTATTGATTATGATCCAGACGCTGAAACATTCCGTGGTGAGTT
CGTAGGCTTAAACGGCGGCGCGGATTTCTATGGTGAAAGCGTCGCTCAATTGGAAGCCGAGGGCGCGAAGTCATTAAGCG
TTTTTTTAGAGATGTGTCAGGAGCGTGGCATTGAGCCTTACAAAAACTTCTCTGGCAAATTCAACGTTCGGATCTCGCCA
GAGGTTCACGCTCGACTTAATGAAATTGCGCTATCGCAAAGCATTAGTTTGAATGCAGCGGTAGAAAATGCAGTCAATGA
CTACATTGCACATTCAGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0R9ME90