Detailed information of TA system TA235666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2121021..2121583
• Replicon: chromosome
• Accession: NZ_CP092075
• Organism: Salmonella enterica strain P281972

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5U2RHV4
• Locus tag: MC377_RS10680
• Protein ID: WP_071686223.1
• Coordinates: 2121305..2121583 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MC377_RS10675
• Protein ID: WP_001240607.1
• Coordinates: 2121021..2121305 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC377_RS10650 (2116689)	2116689..2117411	-	723	WP_048591017.1	SPI-1 type III secretion system guanine nucleotide exchange factor SopE	-
MC377_RS10655 (2117621)	2117621..2117829	+	209	Protein_2091	helix-turn-helix domain-containing protein	-
MC377_RS10660 (2117961)	2117961..2118662	-	702	WP_086126161.1	DUF1076 domain-containing protein	-
MC377_RS10665 (2119486)	2119486..2119881	-	396	Protein_2093	tyrosine-type recombinase/integrase	-
MC377_RS10670 (2120524)	2120524..2120862	+	339	WP_114050305.1	T3SS effector NleG family protein	-
MC377_RS10675 (2121021)	2121021..2121305	-	285	WP_001240607.1	HigA family addiction module antitoxin	Antitoxin
MC377_RS10680 (2121305)	2121305..2121583	-	279	WP_071686223.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MC377_RS10685 (2121835)	2121835..2122095	+	261	WP_002953402.1	hypothetical protein	-
MC377_RS10690 (2122166)	2122166..2122849	-	684	WP_238728147.1	PipA/GogA/GtgA family type III secretion system effector	-
MC377_RS10695 (2123368)	2123368..2123826	+	459	WP_000502123.1	IS200/IS605-like element IS200F family transposase	-
MC377_RS10700 (2123986)	2123986..2124816	+	831	WP_227012499.1	hypothetical protein	-
MC377_RS10705 (2124819)	2124819..2125350	+	532	Protein_2101	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sseI/srfH / sspH2 / sopE2 / sspH2 / sspH1 / sspH2 / sopE2 / sopE2	2000099..2163324	163225
	-	flank	IS/Tn	-	-	2123581..2123826	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10751.24 Da
• Isoelectric Point: 9.4011

>T235666 WP_071686223.1 NZ_CP092075:c2121583-2121305 [Salmonella enterica]
MIKSFRHKGLKKFYQKGDSSGLEQSLVPRLKNRLSMLDAAKTIDFLNQQGYDLHELKGDRAETWSIKVSGNWRLTFIFEN
GDVYVLNLEDYH

Nucleotide

• Length: 279 bp

>T235666 NZ_LR134320:c211113-210598 [Streptococcus mutans]
ATGTTTGACCTTGTTGTTAATTTGATTTTATTGGTTATTGTGATAGGTGGATTTGTCTTCTTAAGATTCTATGCGGATAA
GAAAGGCAAGCGAGAATACGATGAACGCCAGTTACTTATGCAAAAGAAAGCTTATACCAATGCGGCTTGGGTTGTTATGG
GTTTCAATCTTGTTCTTGTGATATGGGGTGAAGTGTTAGCAAAATATATCTCGCTATCCTTTGCAGGTACTGCCAATCTC
TTTCTTATCGTAGGCGTCTTTGTCTGCCACAGTATCTTAAACGATGCTTATTTCACAGCAAGAAAAAACAAAAGATTCCT
CTATGTGTATGCCGTTATTATTGCTATTCAAATTTTCACTGTTTATCAAAATTGGAGTCAAGGCAGCTTTGGTCATGACG
GACATATTTATCTAACAGGAGAAAAAGCAATGAGTTTATTGTTTATCTTGACTTTTGCTGTCATTTTTCTAGTAACTGCT
TATAAAACGATTCAGGATAAGAGAGAGGGAAAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10187.74 Da
• Isoelectric Point: 5.6051

>AT235666 WP_001240607.1 NZ_CP092075:c2121305-2121021 [Salmonella enterica]
MAMFDPAYPGEIIAEILEDQGVSLRQFAVAMNIAPSTASRILSGKVSITPEMAVKLAIVLGGDAESWLNLQNNYSLSQAR
KTVDITHVHRLSFA

Nucleotide

• Length: 285 bp

>AT235666 NZ_LR134320:c210598-210152 [Streptococcus mutans]
ATGAAAATGGAAAAGTCAAATAAACAAGTCATTTACGATGAACGTCAGCAGCAAATTCAACTTAAAAGTTATAGTCTTTC
ATTTTGGTTTGTTATGTTTATACTCTATTTTGCAACTTTTGGAAAGACTGATTTGTTGCTTAATATCGCTTTTTGGGGAG
GACTTGTTCTTAATTTTTGCTACAGTACTCTAAGAGGTGTTGGTCCTTTTGTTGATCCACGTTTTGGAAAAATTGCAAAA
ATCGGTCGTTTGGCCGCTGTTCCCCTTATCTTTTTGGGAATGCTAGTCTTTCTTGTTGCTATCATAATGTCTATTCTAGA
ACATGATAGTTTAAGAGAATCTATCATAAAATGCTCCTACTTAGGCTTATCTGGTTTTTGGCTAATTTGTATGGGAGCAA
GTGTCGTCTACCGTCATTATCTTGATAAGAAAGAAGCAGACAAATGA

Structures

Toxin

• AlphaFold DB: A0A5U2RHV4