Detailed information of TA system TA235641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4951431..4952155
• Replicon: chromosome
• Accession: NZ_CP092070
• Organism: Pectobacterium sp. PL64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7V8TLN0
• Locus tag: HP572_RS22275
• Protein ID: WP_039504225.1
• Coordinates: 4951431..4951742 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HP572_RS22280
• Protein ID: WP_095993507.1
• Coordinates: 4951739..4952155 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP572_RS22260 (HP572_22260)	4947605..4949140	-	1536	WP_240584354.1	carbohydrate porin	-
HP572_RS22265 (HP572_22265)	4949339..4950262	-	924	WP_240584355.1	aminoimidazole riboside kinase	-
HP572_RS22270 (HP572_22270)	4950722..4951273	+	552	WP_240584356.1	polymer-forming cytoskeletal protein	-
HP572_RS22275 (HP572_22275)	4951431..4951742	+	312	WP_039504225.1	type II toxin-antitoxin system HigB family toxin	Toxin
HP572_RS22280 (HP572_22280)	4951739..4952155	+	417	WP_095993507.1	helix-turn-helix domain-containing protein	Antitoxin
HP572_RS22285 (HP572_22285)	4952362..4952820	+	459	WP_014913932.1	hypothetical protein	-
HP572_RS22290 (HP572_22290)	4952915..4954267	-	1353	WP_240584357.1	pyridoxal-dependent decarboxylase	-
HP572_RS22295 (HP572_22295)	4954321..4955565	-	1245	WP_240584358.1	MFS transporter	-
HP572_RS22300 (HP572_22300)	4955719..4956165	+	447	WP_240584359.1	helix-turn-helix domain-containing protein	-
HP572_RS22305 (HP572_22305)	4956175..4956621	+	447	WP_240584360.1	YhbP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12406.28 Da
• Isoelectric Point: 10.0380

>T235641 WP_039504225.1 NZ_CP092070:4951431-4951742 [Pectobacterium sp. PL64]
MHVISRAPFDTATTQFPNQAAALADLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T235641 NZ_LR134315:1286465-1286863 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15391.63 Da
• Isoelectric Point: 4.6581

>AT235641 WP_095993507.1 NZ_CP092070:4951739-4952155 [Pectobacterium sp. PL64]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYENDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILNGERTLTLDHMRALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT235641 NZ_LR134315:1286238-1286465 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A7V8TLN0