Detailed information of TA system TA023561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4146628..4147230
• Replicon: chromosome
• Accession: NC_011993
• Organism: Escherichia coli LF82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U9FTF1
• Locus tag: LF82_RS20505
• Protein ID: WP_000897301.1
• Coordinates: 4146628..4146939 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LF82_RS20510
• Protein ID: WP_000356397.1
• Coordinates: 4146940..4147230 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LF82_RS20480	4142543..4143142	+	600	WP_001314324.1	glucose-1-phosphatase	-
LF82_RS20485	4143136..4144008	+	873	WP_000920762.1	virulence factor BrkB family protein	-
LF82_RS20490	4144005..4144442	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
LF82_RS20495	4144487..4145428	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LF82_RS20500	4145492..4146400	-	909	WP_001331553.1	alpha/beta hydrolase	-
LF82_RS20505	4146628..4146939	+	312	WP_000897301.1	hypothetical protein	Toxin
LF82_RS20510	4146940..4147230	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
LF82_RS20520	4147589..4147867	+	279	WP_001296612.1	hypothetical protein	-
LF82_RS20525	4148263..4148481	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
LF82_RS20530	4148697..4149626	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LF82_RS20535	4149623..4150258	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LF82_RS20540	4150255..4151157	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12150.14 Da
• Isoelectric Point: 8.8941

>T23561 WP_000897301.1 NC_011993:4146628-4146939 [Escherichia coli LF82]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKCAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T23561 NC_011993:4146628-4146939 [Escherichia coli LF82]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAATGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT23561 WP_000356397.1 NC_011993:4146940-4147230 [Escherichia coli LF82]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT23561 NC_011993:4146940-4147230 [Escherichia coli LF82]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9FTF1