Detailed information of TA system TA235589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4869331..4869933
• Replicon: chromosome
• Accession: NZ_CP092042
• Organism: Enterobacter cloacae isolate AVS0889

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E1AN02
• Locus tag: MBA36_RS23295
• Protein ID: WP_013099360.1
• Coordinates: 4869622..4869933 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MBA36_RS23290
• Protein ID: WP_013099359.1
• Coordinates: 4869331..4869621 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA36_RS23275 (MBA36_23275)	4866828..4867730	+	903	WP_013099356.1	formate dehydrogenase subunit beta	-
MBA36_RS23280 (MBA36_23280)	4867727..4868362	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
MBA36_RS23285 (MBA36_23285)	4868359..4869288	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
MBA36_RS23290 (MBA36_23290)	4869331..4869621	-	291	WP_013099359.1	helix-turn-helix domain-containing protein	Antitoxin
MBA36_RS23295 (MBA36_23295)	4869622..4869933	-	312	WP_013099360.1	hypothetical protein	Toxin
MBA36_RS23300 (MBA36_23300)	4870163..4871080	+	918	WP_013099361.1	alpha/beta hydrolase	-
MBA36_RS23305 (MBA36_23305)	4871093..4872034	-	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
MBA36_RS23310 (MBA36_23310)	4872079..4872516	-	438	WP_013099363.1	D-aminoacyl-tRNA deacylase	-
MBA36_RS23315 (MBA36_23315)	4872513..4873394	-	882	WP_013099364.1	virulence factor BrkB family protein	-
MBA36_RS23320 (MBA36_23320)	4873388..4873987	-	600	WP_013099365.1	glucose-1-phosphatase	-
MBA36_RS23325 (MBA36_23325)	4874106..4874906	-	801	WP_013099366.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12210.18 Da
• Isoelectric Point: 9.7550

>T235589 WP_013099360.1 NZ_CP092042:c4869933-4869622 [Enterobacter cloacae]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLATQPDYGDLIQNTGGLRKIRWLSGGRGKRGGVRVIYFHRTHEFEIRLLLIY
RKGIKDDLSAREKAILKKMIERW

Nucleotide

• Length: 312 bp

>T235589 NZ_LR134308:5382216-5382497 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10623.13 Da
• Isoelectric Point: 10.4747

>AT235589 WP_013099359.1 NZ_CP092042:c4869621-4869331 [Enterobacter cloacae]
MDKALFERLTQSIAQMDDIVGGKREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVISALSD

Nucleotide

• Length: 291 bp

>AT235589 NZ_LR134308:5381992-5382219 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A5E1AN02