Detailed information of TA system TA235525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2917104..2917831
• Replicon: chromosome
• Accession: NZ_CP092037
• Organism: Escherichia coli strain MG1655-377GD

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9ZMR4
• Locus tag: MBA16_RS14495
• Protein ID: WP_000550189.1
• Coordinates: 2917517..2917831 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MBA16_RS14490
• Protein ID: WP_000560266.1
• Coordinates: 2917104..2917520 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA16_RS14480 (2912264)	2912264..2914615	+	2352	WP_000695487.1	alpha-glucosidase	-
MBA16_RS14485 (2915041)	2915041..2917059	+	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
MBA16_RS14490 (2917104)	2917104..2917520	-	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
MBA16_RS14495 (2917517)	2917517..2917831	-	315	WP_000550189.1	type II toxin-antitoxin system toxin HigB	Toxin
MBA16_RS14500 (2918115)	2918115..2919251	-	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
MBA16_RS14505 (2919336)	2919336..2919839	+	504	WP_001333820.1	M48 family metallopeptidase	-
MBA16_RS14510 (2919916)	2919916..2920608	+	693	WP_000942548.1	vancomycin high temperature exclusion protein	-
MBA16_RS14515 (2920687)	2920687..2921673	+	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12103.10 Da
• Isoelectric Point: 10.0409

>T235525 WP_000550189.1 NZ_CP092037:c2917831-2917517 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T235525 NZ_LR134299:c1968296-1967721 [Pseudomonas putida]
ATGAAGCACTCGCCCTTCACGGCCATATACGACGCGAATGTACTTTACCCTGCTCCACTGCGTGATTTCCTGATGAACCT
AGCGCTGACGGGCATCTACCGGGCACGTTGGTCAGCGAGCATTCATGACGAGTGGAAGCGCAATCTGCTACTCAACCGAC
CTGACCTCACGCCTGAACATTTGGATCGCACGTCATCGCTGATGGATGCTGCGGTGCCTGATGCACTCGTCACTGACTAC
GATTCACTGGTCGAAGGGCTGGACTTGCCAGATAGGGATGATCGACATGTTCTCGCAGCTGCAATCAAGTGCAACGCTTC
GGTCATCGTCACCTTCAATTTGAAGGATTTTTCCAAGGCGGTTCTTGGTGCCTTCGATATTGAACCCTTGCATCCGGACG
ACTTCATCGCTGACCTTTGGGACCTGGACAAGGCAGCAGTCCTCGAAGCTGCCCAGCGGCAGCGAATATCGCTGAAGAAC
CCACCGCACAGCGTACAGCAGTATCTCGACAGGCTGCTTCAGCAGAAGCTTCCCGAGACAGTGAAACTGCTTTCAGCATT
CAAGTTCCTCCTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT235525 WP_000560266.1 NZ_CP092037:c2917520-2917104 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT235525 NZ_LR134299:c1968760-1968293 [Pseudomonas putida]
ATGACCACCGCCAACCTCTCTAGCCATGTGCTTCCTGACGAGAAAGAAATTGCCGTGGCAGTCGAGTCAAGCCGCACGCT
GGCAGCGTTCCTCTCGACAAAATCAGATACCCAACGGATCGAACTGCTCAACGAGGAAAATCAGCGCCAGGTTGTTGAAG
TACCTACTTTCGCCCTACGCCTATTCGGCGAAATTCTCAGCGAACTGGCCTTGGGTAACTCAGTCAAAGTCGTTCCAATC
CACGCCGAACTAACTACCCAGGAAGCAGCAGACCTGCTTAACGTCTCCCGGCCGCACTTGGTAAAGCTGCTCGACGAAGC
GGTGATTCCGCACACCAAGACAGGCCGCCATCGTCGTGTCAAATTCTCAGACCTGGTTGCCTACAAGGACAAACGTGATG
GGGTGAGCCGCGCTGCGATAGATGCGCTGGCAGCCCAAGCTCAGGAATTGAAGATGGGGTACGAATGA

Structures

Toxin

• PDB: 6KMQ
• PDB: 5IFG
• PDB: 5YCL
• PDB: 6KML