Detailed information of TA system TA235524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2856884..2857577
• Replicon: chromosome
• Accession: NZ_CP092037
• Organism: Escherichia coli strain MG1655-377GD

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: MBA16_RS14205
• Protein ID: WP_000415584.1
• Coordinates: 2857281..2857577 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: MBA16_RS14200
• Protein ID: WP_000650107.1
• Coordinates: 2856884..2857279 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MBA16_RS14190 (2852748)	2852748..2855006	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
MBA16_RS14195 (2855144)	2855144..2856751	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MBA16_RS14200 (2856884)	2856884..2857279	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
MBA16_RS14205 (2857281)	2857281..2857577	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
MBA16_RS14210 (2857782)	2857782..2858264	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
MBA16_RS14215 (2858317)	2858317..2858709	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MBA16_RS14220 (2858861)	2858861..2859520	+	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
MBA16_RS14225 (2859517)	2859517..2860866	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
MBA16_RS14230 (2860912)	2860912..2861244	-	333	WP_000917684.1	DUF2645 family protein	-
MBA16_RS14235 (2861563)	2861563..2862144	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
MBA16_RS14240 (2862175)	2862175..2862489	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T235524 WP_000415584.1 NZ_CP092037:c2857577-2857281 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T235524 NZ_LR134299:1908882-1909178 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT235524 WP_000650107.1 NZ_CP092037:c2857279-2856884 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT235524 NZ_LR134299:1909181-1909582 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65