Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
Location | 242469..243163 | Replicon | chromosome |
Accession | NZ_CP092037 | ||
Organism | Escherichia coli strain MG1655-377GD |
Toxin (Protein)
Gene name | yafO | Uniprot ID | Q47157 |
Locus tag | MBA16_RS01150 | Protein ID | WP_001263489.1 |
Coordinates | 242765..243163 (+) | Length | 133 a.a. |
Antitoxin (Protein)
Gene name | yafN | Uniprot ID | S1QAE3 |
Locus tag | MBA16_RS01145 | Protein ID | WP_000554758.1 |
Coordinates | 242469..242762 (+) | Length | 98 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
MBA16_RS01125 (238101) | 238101..238598 | + | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
MBA16_RS01130 (238822) | 238822..240534 | - | 1713 | Protein_218 | flagellar biosynthesis protein FlhA | - |
MBA16_RS01135 (240506) | 240506..241291 | + | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
MBA16_RS01140 (241362) | 241362..242417 | + | 1056 | WP_001226164.1 | DNA polymerase IV | - |
MBA16_RS01145 (242469) | 242469..242762 | + | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
MBA16_RS01150 (242765) | 242765..243163 | + | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
MBA16_RS01155 (243173) | 243173..243625 | + | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
MBA16_RS01160 (243943) | 243943..244149 | + | 207 | Protein_224 | RtcB family protein | - |
MBA16_RS01165 (244145) | 244145..244666 | + | 522 | Protein_225 | peptide chain release factor H | - |
MBA16_RS01170 (244723) | 244723..246180 | - | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
MBA16_RS01175 (246441) | 246441..246899 | + | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
- (247495) | 247495..247575 | + | 81 | NuclAT_12 | - | - |
- (247495) | 247495..247575 | + | 81 | NuclAT_12 | - | - |
- (247495) | 247495..247575 | + | 81 | NuclAT_12 | - | - |
- (247495) | 247495..247575 | + | 81 | NuclAT_12 | - | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T235502 WP_001263489.1 NZ_CP092037:242765-243163 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T235502 NZ_LR134295:c4233546-4233373 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT235502 WP_000554758.1 NZ_CP092037:242469-242762 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT235502 NZ_LR134295:c4233870-4233574 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A090J8B1 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | S1QAE3 |