Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 242469..243163 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MBA16_RS01150 Protein ID WP_001263489.1
Coordinates 242765..243163 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MBA16_RS01145 Protein ID WP_000554758.1
Coordinates 242469..242762 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS01125 (238101) 238101..238598 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MBA16_RS01130 (238822) 238822..240534 - 1713 Protein_218 flagellar biosynthesis protein FlhA -
MBA16_RS01135 (240506) 240506..241291 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MBA16_RS01140 (241362) 241362..242417 + 1056 WP_001226164.1 DNA polymerase IV -
MBA16_RS01145 (242469) 242469..242762 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MBA16_RS01150 (242765) 242765..243163 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MBA16_RS01155 (243173) 243173..243625 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
MBA16_RS01160 (243943) 243943..244149 + 207 Protein_224 RtcB family protein -
MBA16_RS01165 (244145) 244145..244666 + 522 Protein_225 peptide chain release factor H -
MBA16_RS01170 (244723) 244723..246180 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MBA16_RS01175 (246441) 246441..246899 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (247495) 247495..247575 + 81 NuclAT_12 - -
- (247495) 247495..247575 + 81 NuclAT_12 - -
- (247495) 247495..247575 + 81 NuclAT_12 - -
- (247495) 247495..247575 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T235502 WP_001263489.1 NZ_CP092037:242765-243163 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T235502 NZ_LR134295:c4233546-4233373 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT235502 WP_000554758.1 NZ_CP092037:242469-242762 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT235502 NZ_LR134295:c4233870-4233574 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References