Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 2785168..2785690 Replicon chromosome
Accession NZ_CP092033
Organism Lacticaseibacillus rhamnosus strain VHProbi Y39

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag LT346_RS12945 Protein ID WP_005687756.1
Coordinates 2785430..2785690 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag LT346_RS12940 Protein ID WP_005687754.1
Coordinates 2785168..2785437 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT346_RS12925 (LT346_12925) 2780655..2782523 - 1869 WP_014571687.1 HTH domain-containing protein -
LT346_RS12930 (LT346_12930) 2782550..2783350 - 801 WP_005690776.1 SDR family oxidoreductase -
LT346_RS12935 (LT346_12935) 2783956..2784972 - 1017 WP_019728331.1 IS30 family transposase -
LT346_RS12940 (LT346_12940) 2785168..2785437 + 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
LT346_RS12945 (LT346_12945) 2785430..2785690 + 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
LT346_RS12950 (LT346_12950) 2785901..2786629 - 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
LT346_RS12955 (LT346_12955) 2786826..2787647 + 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
LT346_RS12960 (LT346_12960) 2787714..2788601 - 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
LT346_RS12965 (LT346_12965) 2788786..2789565 - 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
LT346_RS12970 (LT346_12970) 2789633..2790274 - 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T235499 WP_005687756.1 NZ_CP092033:2785430-2785690 [Lacticaseibacillus rhamnosus]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T235499 NZ_LR134295:3682328-3682606 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT235499 WP_005687754.1 NZ_CP092033:2785168-2785437 [Lacticaseibacillus rhamnosus]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT235499 NZ_LR134295:3682065-3682325 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References