Detailed information of TA system TA235493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3206053..3206679
• Replicon: chromosome
• Accession: NZ_CP092014
• Organism: Edwardsiella ictaluri strain 13 TAL-140 K3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A411H7J1
• Locus tag: MAY91_RS15580
• Protein ID: WP_015871332.1
• Coordinates: 3206350..3206679 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAY91_RS15575
• Protein ID: WP_278068344.1
• Coordinates: 3206053..3206334 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAY91_RS15540 (MAY91_15480)	3201844..3203226	+	1383	WP_049640642.1	replicative DNA helicase	-
MAY91_RS15545 (MAY91_15485)	3203256..3203603	+	348	WP_015871339.1	hypothetical protein	-
MAY91_RS15550 (MAY91_15490)	3203713..3204357	+	645	WP_049640643.1	DUF1367 family protein	-
MAY91_RS15555 (MAY91_15495)	3204360..3204671	+	312	WP_049640644.1	DUF1364 family protein	-
MAY91_RS15560 (MAY91_15500)	3204721..3205071	+	351	WP_049640645.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MAY91_RS15565 (MAY91_15505)	3205073..3205372	+	300	WP_049640646.1	XRE family transcriptional regulator	-
MAY91_RS15570 (MAY91_15510)	3205446..3205958	-	513	WP_015871334.1	DUF2442 domain-containing protein	-
MAY91_RS15575 (MAY91_15515)	3206053..3206334	-	282	WP_278068344.1	helix-turn-helix transcriptional regulator	Antitoxin
MAY91_RS15580 (MAY91_15520)	3206350..3206679	-	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAY91_RS15585 (MAY91_15525)	3207125..3207895	+	771	WP_049640647.1	Rha family transcriptional regulator	-
MAY91_RS15590 (MAY91_15530)	3208024..3208671	+	648	WP_278068345.1	hypothetical protein	-
MAY91_RS15595 (MAY91_15535)	3208713..3209363	+	651	WP_278068346.1	Fic family protein	-
MAY91_RS15600 (MAY91_15540)	3209428..3210246	+	819	WP_049640649.1	hypothetical protein	-
MAY91_RS15605 (MAY91_15545)	3210352..3210596	+	245	Protein_2990	transposase	-
MAY91_RS15610 (MAY91_15550)	3210728..3210958	+	231	WP_015871327.1	ParD-like family protein	-
MAY91_RS15615 (MAY91_15555)	3210933..3211262	+	330	WP_049640650.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3179775..3237664	57889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-85)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12771.78 Da
• Isoelectric Point: 9.6247

>T235493 WP_015871332.1 NZ_CP092014:c3206679-3206350 [Edwardsiella ictaluri]
MNYTIEYYSEEVRLEVDQLPMGMRVRYQHLVERMEIYGSNLGEPHTSPFGDGLFELRIKGSDGIARVFYCTLTGKRIVML
HSFIKKTQKTPSAERKKAETRMKEVKHGW

Nucleotide

• Length: 330 bp

>T235493 NZ_LR134295:c2415906-2415730 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10267.73 Da
• Isoelectric Point: 6.4690

>AT235493 WP_278068344.1 NZ_CP092014:c3206334-3206053 [Edwardsiella ictaluri]
MTHDEMAAKWMEDPAFKAEYDAIEEEYALLDEMLAARKNAGLTQAQVAERMGTKATAITRMESNLASGQSGPSFATLKKF
AHATGKKLQIRFV

Nucleotide

• Length: 282 bp

>AT235493 NZ_LR134295:c2415684-2415268 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A411H7J1