Detailed information of TA system TA235486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4642445..4643047
• Replicon: chromosome
• Accession: NZ_CP092012
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N12-0259

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: MA624_RS22485
• Protein ID: WP_023138876.1
• Coordinates: 4642736..4643047 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA624_RS22480
• Protein ID: WP_000362052.1
• Coordinates: 4642445..4642735 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA624_RS22465 (4639938)	4639938..4640840	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA624_RS22470 (4640837)	4640837..4641472	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA624_RS22475 (4641469)	4641469..4642398	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA624_RS22480 (4642445)	4642445..4642735	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
MA624_RS22485 (4642736)	4642736..4643047	-	312	WP_023138876.1	hypothetical protein	Toxin
MA624_RS22490 (4643265)	4643265..4644194	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA624_RS22495 (4644280)	4644280..4644591	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MA624_RS22500 (4644588)	4644588..4645034	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MA624_RS22505 (4645049)	4645049..4645990	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA624_RS22510 (4646035)	4646035..4646472	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA624_RS22515 (4646469)	4646469..4647341	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA624_RS22520 (4647335)	4647335..4647934	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T235486 WP_023138876.1 NZ_CP092012:c4643047-4642736 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T235486 NZ_LR134295:c606523-606059 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT235486 WP_000362052.1 NZ_CP092012:c4642735-4642445 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT235486 NZ_LR134295:c606858-606523 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCGAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A603E470