Detailed information of TA system TA235466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4609711..4610465
• Replicon: chromosome
• Accession: NZ_CP092009
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N12-0931

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: MA811_RS22240
• Protein ID: WP_000558166.1
• Coordinates: 4609711..4610022 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA811_RS22245
• Protein ID: WP_001259011.1
• Coordinates: 4610019..4610465 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA811_RS22210 (4605369)	4605369..4606271	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA811_RS22215 (4606268)	4606268..4606903	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA811_RS22220 (4606900)	4606900..4607829	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA811_RS22225 (4607876)	4607876..4608166	-	291	WP_000362052.1	DNA-binding transcriptional regulator	-
MA811_RS22230 (4608167)	4608167..4608478	-	312	WP_023138876.1	hypothetical protein	-
MA811_RS22235 (4608696)	4608696..4609625	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA811_RS22240 (4609711)	4609711..4610022	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
MA811_RS22245 (4610019)	4610019..4610465	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
MA811_RS22250 (4610480)	4610480..4611421	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA811_RS22255 (4611466)	4611466..4611903	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA811_RS22260 (4611900)	4611900..4612772	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA811_RS22265 (4612766)	4612766..4613365	-	600	WP_000965695.1	glucose-1-phosphatase	-
MA811_RS22270 (4613556)	4613556..4614359	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
MA811_RS22275 (4614393)	4614393..4615289	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T235466 WP_000558166.1 NZ_CP092009:4609711-4610022 [Salmonella enterica subsp. enterica serovar Kentucky]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T235466 NZ_LR134280:c3465056-3464772 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT235466 WP_001259011.1 NZ_CP092009:4610019-4610465 [Salmonella enterica subsp. enterica serovar Kentucky]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT235466 NZ_LR134280:c3465288-3465046 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90