Detailed information of TA system TA235465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4607876..4608478
• Replicon: chromosome
• Accession: NZ_CP092009
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N12-0931

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: MA811_RS22230
• Protein ID: WP_023138876.1
• Coordinates: 4608167..4608478 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA811_RS22225
• Protein ID: WP_000362052.1
• Coordinates: 4607876..4608166 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA811_RS22210 (4605369)	4605369..4606271	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA811_RS22215 (4606268)	4606268..4606903	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA811_RS22220 (4606900)	4606900..4607829	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA811_RS22225 (4607876)	4607876..4608166	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
MA811_RS22230 (4608167)	4608167..4608478	-	312	WP_023138876.1	hypothetical protein	Toxin
MA811_RS22235 (4608696)	4608696..4609625	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA811_RS22240 (4609711)	4609711..4610022	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MA811_RS22245 (4610019)	4610019..4610465	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MA811_RS22250 (4610480)	4610480..4611421	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA811_RS22255 (4611466)	4611466..4611903	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA811_RS22260 (4611900)	4611900..4612772	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA811_RS22265 (4612766)	4612766..4613365	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T235465 WP_023138876.1 NZ_CP092009:c4608478-4608167 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T235465 NZ_LR134280:c3355049-3354516 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT235465 WP_000362052.1 NZ_CP092009:c4608166-4607876 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT235465 NZ_LR134280:c3355326-3355060 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A603E470