Detailed information of TA system TA235444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4644853..4645455
• Replicon: chromosome
• Accession: NZ_CP092004
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N14-1660

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: MA620_RS22500
• Protein ID: WP_023138876.1
• Coordinates: 4645144..4645455 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA620_RS22495
• Protein ID: WP_000362052.1
• Coordinates: 4644853..4645143 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA620_RS22480 (4642346)	4642346..4643248	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA620_RS22485 (4643245)	4643245..4643880	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA620_RS22490 (4643877)	4643877..4644806	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA620_RS22495 (4644853)	4644853..4645143	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
MA620_RS22500 (4645144)	4645144..4645455	-	312	WP_023138876.1	hypothetical protein	Toxin
MA620_RS22505 (4645673)	4645673..4646602	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA620_RS22510 (4646688)	4646688..4646999	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MA620_RS22515 (4646996)	4646996..4647442	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MA620_RS22520 (4647457)	4647457..4648398	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA620_RS22525 (4648443)	4648443..4648880	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA620_RS22530 (4648877)	4648877..4649749	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA620_RS22535 (4649743)	4649743..4650342	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T235444 WP_023138876.1 NZ_CP092004:c4645455-4645144 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T235444 NZ_LR134270:2172583-2172686 [Escherichia marmotae]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT235444 WP_000362052.1 NZ_CP092004:c4645143-4644853 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT235444 NZ_LR134270:c2172700-2172455 [Escherichia marmotae]
CTGGCTTTATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCGCG
TAAAGTGGTCAGAAAAAAAGCGCGTTTATCATCCTCTAAAATGTTAAAAACCGCCCATTCTGGTGAAAGCACCCGGGCGG
TTTTTT

Structures

Toxin

• AlphaFold DB: A0A603E470