Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1517417..1517979 Replicon chromosome
Accession NC_011993
Organism Escherichia coli LF82

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag LF82_RS07605 Protein ID WP_000605675.1
Coordinates 1517701..1517979 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag LF82_RS07600 Protein ID WP_000781370.1
Coordinates 1517417..1517701 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LF82_RS07585 1512598..1515645 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
LF82_RS07590 1515658..1516542 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
LF82_RS07595 1516535..1517188 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
LF82_RS07600 1517417..1517701 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
LF82_RS07605 1517701..1517979 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LF82_RS07610 1518165..1519175 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
LF82_RS07615 1519309..1521006 - 1698 WP_000433464.1 malate dehydrogenase -
LF82_RS07620 1521163..1521300 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
LF82_RS07625 1521402..1521617 - 216 WP_000495766.1 biofilm-dependent modulation protein -
LF82_RS07630 1521962..1522393 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T23544 WP_000605675.1 NC_011993:c1517979-1517701 [Escherichia coli LF82]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T23544 NC_011993:c1517979-1517701 [Escherichia coli LF82]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT23544 WP_000781370.1 NC_011993:c1517701-1517417 [Escherichia coli LF82]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT23544 NC_011993:c1517701-1517417 [Escherichia coli LF82]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References