Detailed information of TA system TA235424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4581434..4582188
• Replicon: chromosome
• Accession: NZ_CP091999
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N16-1393

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: MA802_RS22080
• Protein ID: WP_000558166.1
• Coordinates: 4581434..4581745 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA802_RS22085
• Protein ID: WP_001259011.1
• Coordinates: 4581742..4582188 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA802_RS22050 (4577092)	4577092..4577994	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA802_RS22055 (4577991)	4577991..4578626	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA802_RS22060 (4578623)	4578623..4579552	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA802_RS22065 (4579599)	4579599..4579889	-	291	WP_000362052.1	DNA-binding transcriptional regulator	-
MA802_RS22070 (4579890)	4579890..4580201	-	312	WP_023138876.1	hypothetical protein	-
MA802_RS22075 (4580419)	4580419..4581348	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA802_RS22080 (4581434)	4581434..4581745	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
MA802_RS22085 (4581742)	4581742..4582188	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
MA802_RS22090 (4582203)	4582203..4583144	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA802_RS22095 (4583189)	4583189..4583626	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA802_RS22100 (4583623)	4583623..4584495	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA802_RS22105 (4584489)	4584489..4585088	-	600	WP_000965695.1	glucose-1-phosphatase	-
MA802_RS22110 (4585279)	4585279..4586082	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
MA802_RS22115 (4586116)	4586116..4587012	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T235424 WP_000558166.1 NZ_CP091999:4581434-4581745 [Salmonella enterica subsp. enterica serovar Kentucky]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T235424 NZ_LR134264:965970-966176 [Staphylococcus simulans]
ATGCATAGTAGTGTGATTCAAATCAGTGGTATGGAAACTGAAGAACAACAGCATATTTTGCAAGAACATTTGCAGCAAAT
GATTGGTATGTCAGAAGTGCAAGTGGATCTGAAATCACAAGAAGTGAAAGCGAGTTACCAGACACCAATCAGTTTAAACA
ATTTGGAAAAAGAAATTTATGATCAAGGTTACCAAATATTAAATTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT235424 WP_001259011.1 NZ_CP091999:4581742-4582188 [Salmonella enterica subsp. enterica serovar Kentucky]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT235424 NZ_LR134264:965662-965955 [Staphylococcus simulans]
ATGACGCAAGAAGAACGCGCACATCATTCAGAAAAAATAAAATCTAACCTTAAAGCACGACTGAATCGTATAGAAGGACA
AGTCAGAGCGATTAATCGCATGATAGATGAAGATGTTTATTGTGATGATGTTTTAACACAAATCAGAGCGACACGTTCAG
CTTTGAATAGTGTTGCTGTAAAATTGCTGGATCATCATATGAAAGGCTGTATTTTGCATAAAGCTGAAGATGGACAAGCA
GAAGCTGCAATGGAAGAATTATTAGTTACCGTCCAAAAATTAATTAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90