Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 288390..289084 Replicon chromosome
Accession NC_011993
Organism Escherichia coli LF82

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag LF82_RS01340 Protein ID WP_001263501.1
Coordinates 288686..289084 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag LF82_RS01335 Protein ID WP_000554755.1
Coordinates 288390..288683 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LF82_RS01315 283828..284325 + 498 WP_000006238.1 REP-associated tyrosine transposase RayT -
LF82_RS25470 284320..284664 - 345 WP_137486338.1 hypothetical protein -
LF82_RS01320 284743..286455 - 1713 Protein_254 flagellar biosynthesis protein FlhA -
LF82_RS01325 286427..287212 + 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
LF82_RS01330 287283..288338 + 1056 WP_001226167.1 DNA polymerase IV -
LF82_RS01335 288390..288683 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LF82_RS01340 288686..289084 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LF82_RS01345 289094..289546 + 453 WP_001059867.1 GNAT family N-acetyltransferase -
LF82_RS01350 289863..291002 + 1140 WP_000521551.1 RNA ligase RtcB family protein -
LF82_RS01355 290999..291613 + 615 WP_000602135.1 peptide chain release factor H -
LF82_RS01360 291670..293127 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
LF82_RS01365 293388..293846 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T23542 WP_001263501.1 NC_011993:288686-289084 [Escherichia coli LF82]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T23542 NC_011993:288686-289084 [Escherichia coli LF82]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT23542 WP_000554755.1 NC_011993:288390-288683 [Escherichia coli LF82]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT23542 NC_011993:288390-288683 [Escherichia coli LF82]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGGTATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References