Detailed information of TA system TA235401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4455754..4456356
• Replicon: chromosome
• Accession: NZ_CP091998
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N18-2092

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: MA840_RS21635
• Protein ID: WP_023138876.1
• Coordinates: 4456045..4456356 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA840_RS21630
• Protein ID: WP_000362052.1
• Coordinates: 4455754..4456044 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA840_RS21615 (4453247)	4453247..4454149	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA840_RS21620 (4454146)	4454146..4454781	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA840_RS21625 (4454778)	4454778..4455707	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA840_RS21630 (4455754)	4455754..4456044	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
MA840_RS21635 (4456045)	4456045..4456356	-	312	WP_023138876.1	hypothetical protein	Toxin
MA840_RS21640 (4456574)	4456574..4457503	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA840_RS21645 (4457589)	4457589..4457900	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MA840_RS21650 (4457897)	4457897..4458343	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MA840_RS21655 (4458358)	4458358..4459299	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA840_RS21660 (4459344)	4459344..4459781	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA840_RS21665 (4459778)	4459778..4460650	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA840_RS21670 (4460644)	4460644..4461243	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T235401 WP_023138876.1 NZ_CP091998:c4456356-4456045 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T235401 NZ_LR134247:c4490307-4490134 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT235401 WP_000362052.1 NZ_CP091998:c4456044-4455754 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT235401 NZ_LR134247:c4490631-4490335 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A603E470