Detailed information of TA system TA235381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4213515..4214117
• Replicon: chromosome
• Accession: NZ_CP091997
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain N20-2289

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A603E470
• Locus tag: MA641_RS20480
• Protein ID: WP_023138876.1
• Coordinates: 4213806..4214117 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MA641_RS20475
• Protein ID: WP_000362052.1
• Coordinates: 4213515..4213805 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MA641_RS20460 (4211008)	4211008..4211910	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
MA641_RS20465 (4211907)	4211907..4212542	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MA641_RS20470 (4212539)	4212539..4213468	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MA641_RS20475 (4213515)	4213515..4213805	-	291	WP_000362052.1	DNA-binding transcriptional regulator	Antitoxin
MA641_RS20480 (4213806)	4213806..4214117	-	312	WP_023138876.1	hypothetical protein	Toxin
MA641_RS20485 (4214335)	4214335..4215264	+	930	WP_001127698.1	alpha/beta hydrolase	-
MA641_RS20490 (4215350)	4215350..4215661	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MA641_RS20495 (4215658)	4215658..4216104	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MA641_RS20500 (4216119)	4216119..4217060	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MA641_RS20505 (4217105)	4217105..4217542	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
MA641_RS20510 (4217539)	4217539..4218411	-	873	WP_000921423.1	virulence factor BrkB family protein	-
MA641_RS20515 (4218405)	4218405..4219004	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12356.30 Da
• Isoelectric Point: 9.4460

>T235381 WP_023138876.1 NZ_CP091997:c4214117-4213806 [Salmonella enterica subsp. enterica serovar Kentucky]
MQFIETELFTEDVKKLLDEDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T235381 NZ_LR134244:1926537-1926722 [Staphylococcus warneri]
ATGAGCTTGCATTTTACAATTCTTTTTTGGCTTTCACTTATATTTATCGTAGCTGGTGCGATTATTCTAGCTATCATGCT
TAAAACTAAAAAAGAATCTAAGAAAGAATCTTATCTTGGTTTCACCATTGTATTCTTCATTTTTGGATTAGCTATGTTGA
TATATACATTGTTATTCGGACTCTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10970.65 Da
• Isoelectric Point: 11.0303

>AT235381 WP_000362052.1 NZ_CP091997:c4213805-4213515 [Salmonella enterica subsp. enterica serovar Kentucky]
MDKVLFERLTQSMSQMNEIIEGTRKPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT235381 NZ_LR134244:1926260-1926517 [Staphylococcus warneri]
ATGAAACTATACATTATACATGCAATCAATATTATTTTAACGATATTATTTATTATTTTTAATGTAATTATTACTTATAA
TGCAAACCTAGACGATACCTTATGGTTAGTGCCTGGATTAATTGTATGCGGTTTGATTATGATGATTAGTTTTGCTATTG
CTATAACTAAAAAGGACTTGTTAAGTGAAGTTCTTTTCTTTATTAATATTATTCTTACACTGTACTACATTTATCCAATT
TTTTATGATTTTCTTTAA

Structures

Toxin

• AlphaFold DB: A0A603E470