Detailed information of TA system TA235308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2145013..2145688
• Replicon: chromosome
• Accession: NZ_CP091977
• Organism: Aromatoleum aromaticum strain pCyN1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L8M16_RS10305
• Protein ID: WP_211160130.1
• Coordinates: 2145013..2145384 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L8M16_RS10310
• Protein ID: WP_011239183.1
• Coordinates: 2145377..2145688 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8M16_RS10270	2141111..2142421	-	1311	WP_256469908.1	IS4-like element ISAzo5 family transposase	-
L8M16_RS10275	2142802..2143146	+	345	WP_168955037.1	DUF86 domain-containing protein	-
L8M16_RS10280	2143136..2143522	-	387	WP_041646537.1	type II toxin-antitoxin system VapC family toxin	-
L8M16_RS10285	2143519..2143767	-	249	WP_168955038.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
L8M16_RS10290	2143937..2144272	+	336	WP_011239179.1	H-NS histone family protein	-
L8M16_RS10295	2144253..2144732	+	480	WP_011239180.1	VanZ family protein	-
L8M16_RS10300	2144747..2145013	+	267	WP_168955039.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
L8M16_RS10305	2145013..2145384	+	372	WP_211160130.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L8M16_RS10310	2145377..2145688	+	312	WP_011239183.1	helix-turn-helix transcriptional regulator	Antitoxin
L8M16_RS10315	2145730..2146173	+	444	WP_011239184.1	hypothetical protein	-
L8M16_RS10320	2146424..2147983	+	1560	WP_251199588.1	IS21 family transposase	-
L8M16_RS10325	2148006..2148740	+	735	WP_011236010.1	IS21-like element ISAzo4 family helper ATPase IstB	-
L8M16_RS10330	2149165..2149452	+	288	WP_041646540.1	DUF1778 domain-containing protein	-
L8M16_RS10335	2149449..2149940	+	492	WP_168954188.1	GNAT family N-acetyltransferase	-
L8M16_RS10340	2150024..2150314	+	291	WP_011239187.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tviB / bplB / cap8E	2087409..2149940	62531
	-	inside	IScluster/Tn	-	-	2136643..2148740	12097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (29-118)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-98)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 14379.77 Da
• Isoelectric Point: 9.0905

>T235308 WP_211160130.1 NZ_CP091977:2145013-2145384 [Aromatoleum aromaticum]
MPFVLDVSKIANIQDMNWTVSYFNERVREEITKWPVGIYADFLRLIFLMEEHGADLRLPHSRAMGDGLFELRCKGEEGIG
RVFYCTLVGRQIVILHSFIKKTQETPDRELKTARKRLKEVKHG

Nucleotide

• Length: 372 bp

>T235308 NZ_LR134236:c4287530-4287357 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11516.26 Da
• Isoelectric Point: 10.2119

>AT235308 WP_011239183.1 NZ_CP091977:2145377-2145688 [Aromatoleum aromaticum]
MDKEHFKPVRLDTKAELARAMQRPGFKAAWDGLEEEYAALNELLQARKQAGLTQEEVAARMGTTKSAVSRLEASIRSESH
SPSFATLRKYAHACGKRLVIKMV

Nucleotide

• Length: 312 bp

>AT235308 NZ_LR134236:c4287854-4287558 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG