Detailed information of TA system TA235267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3761484..3762178
• Replicon: chromosome
• Accession: NZ_CP091925
• Organism: Escherichia coli strain JNQH497

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KIF99_RS18510
• Protein ID: WP_001263489.1
• Coordinates: 3761484..3761882 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KIF99_RS18515
• Protein ID: WP_000554758.1
• Coordinates: 3761885..3762178 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3757072)	3757072..3757152	-	81	NuclAT_12	-	-
- (3757072)	3757072..3757152	-	81	NuclAT_12	-	-
- (3757072)	3757072..3757152	-	81	NuclAT_12	-	-
- (3757072)	3757072..3757152	-	81	NuclAT_12	-	-
KIF99_RS18485 (3757748)	3757748..3758206	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KIF99_RS18490 (3758467)	3758467..3759924	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KIF99_RS18495 (3759981)	3759981..3760502	-	522	Protein_3636	peptide chain release factor H	-
KIF99_RS18500 (3760498)	3760498..3760704	-	207	Protein_3637	RtcB family protein	-
KIF99_RS18505 (3761022)	3761022..3761474	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KIF99_RS18510 (3761484)	3761484..3761882	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KIF99_RS18515 (3761885)	3761885..3762178	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KIF99_RS18520 (3762230)	3762230..3763285	-	1056	WP_001226164.1	DNA polymerase IV	-
KIF99_RS18525 (3763356)	3763356..3764279	-	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
KIF99_RS18530 (3764282)	3764282..3765145	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
KIF99_RS18535 (3765158)	3765158..3765874	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
KIF99_RS18540 (3765894)	3765894..3766361	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T235267 WP_001263489.1 NZ_CP091925:c3761882-3761484 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T235267 NZ_LR134234:3794272-3794622 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT235267 WP_000554758.1 NZ_CP091925:c3762178-3761885 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT235267 NZ_LR134234:3794027-3794278 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3