Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 646777..647504 Replicon chromosome
Accession NZ_CP091925
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KIF99_RS03155 Protein ID WP_000550189.1
Coordinates 646777..647091 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KIF99_RS03160 Protein ID WP_000560266.1
Coordinates 647088..647504 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS03135 (642931) 642931..643917 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
KIF99_RS03140 (643996) 643996..644688 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KIF99_RS03145 (644769) 644769..645272 - 504 WP_001326618.1 M48 family metallopeptidase -
KIF99_RS03150 (645357) 645357..646493 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KIF99_RS03155 (646777) 646777..647091 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KIF99_RS03160 (647088) 647088..647504 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KIF99_RS03165 (647549) 647549..649567 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KIF99_RS03170 (649993) 649993..652344 - 2352 WP_000695485.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T235248 WP_000550189.1 NZ_CP091925:646777-647091 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T235248 NZ_LR134231:2594004-2594111 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT235248 WP_000560266.1 NZ_CP091925:647088-647504 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT235248 NZ_LR134231:c2593957-2593891 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References