Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603427..604226 Replicon chromosome
Accession NZ_CP091925
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KIF99_RS02930 Protein ID WP_000347273.1
Coordinates 603427..603891 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KIF99_RS02935 Protein ID WP_001307405.1
Coordinates 603891..604226 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS02900 (598428) 598428..598862 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KIF99_RS02905 (598880) 598880..599758 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KIF99_RS02910 (599748) 599748..600527 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KIF99_RS02915 (600538) 600538..601011 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KIF99_RS02920 (601034) 601034..602314 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KIF99_RS02925 (602563) 602563..603372 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KIF99_RS02930 (603427) 603427..603891 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KIF99_RS02935 (603891) 603891..604226 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KIF99_RS02940 (604375) 604375..605946 - 1572 WP_001273753.1 galactarate dehydratase -
KIF99_RS02945 (606321) 606321..607655 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KIF99_RS02950 (607671) 607671..608441 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T235247 WP_000347273.1 NZ_CP091925:c603891-603427 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T235247 NZ_LR134231:2593469-2593576 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT235247 WP_001307405.1 NZ_CP091925:c604226-603891 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT235247 NZ_LR134231:c2593422-2593356 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References