Detailed information of TA system TA235240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3239825..3240431
• Replicon: chromosome
• Accession: NZ_CP091913
• Organism: Nostoc sp. UHCC 0870

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L6494_RS13685
• Protein ID: WP_237988273.1
• Coordinates: 3239825..3240109 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L6494_RS13690
• Protein ID: WP_237988274.1
• Coordinates: 3240123..3240431 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6494_RS13675 (L6494_13670)	3235138..3238674	+	3537	WP_237988271.1	methionine synthase	-
L6494_RS13680 (L6494_13675)	3238824..3239759	-	936	WP_237988272.1	pyridoxal-phosphate dependent enzyme	-
L6494_RS13685 (L6494_13680)	3239825..3240109	+	285	WP_237988273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6494_RS13690 (L6494_13685)	3240123..3240431	+	309	WP_237988274.1	HigA family addiction module antitoxin	Antitoxin
L6494_RS13695 (L6494_13690)	3240625..3241797	+	1173	WP_237988275.1	hypothetical protein	-
L6494_RS13700 (L6494_13695)	3241898..3242783	+	886	Protein_2712	tetratricopeptide repeat protein	-
L6494_RS13705 (L6494_13700)	3243313..3244638	+	1326	WP_237988276.1	DUF58 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (14-94)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-89)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.37 Da
• Isoelectric Point: 4.7961

>T235240 WP_237988273.1 NZ_CP091913:3239825-3240109 [Nostoc sp. UHCC 0870]
VIVSFKSEETKLIFDGFISAEYPPDIQKVALRKLLMLNAATSIQDLRVPPGNRLEKLVGDRQGQYSIRINDQWRICFVWS
NENNADEVEIVDYH

Nucleotide

• Length: 285 bp

>T235240 NZ_LR134231:966622-966957 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11680.34 Da
• Isoelectric Point: 6.9738

>AT235240 WP_237988274.1 NZ_CP091913:3240123-3240431 [Nostoc sp. UHCC 0870]
MNSNCLPNIHPGEILQLEFLEPLNITPYRLSKDIGVTQTRIGEILSGKRSITADTALRLSRYFGNSPQFWLNLQMQYDLR
QAWVEKADVYNQIPVNPFNSVA

Nucleotide

• Length: 309 bp

>AT235240 NZ_LR134231:966374-966622 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA