Detailed information of TA system TA235233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 75611..76136
• Replicon: plasmid p1
• Accession: NZ_CP091907
• Organism: Enterococcus faecalis strain 2-1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: L5I26_RS13550
• Protein ID: WP_049098536.1
• Coordinates: 75858..76136 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A1L8R1L1
• Locus tag: L5I26_RS13545
• Protein ID: WP_071865807.1
• Coordinates: 75611..75871 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I26_RS13515 (70612)	70612..70914	+	303	WP_142425811.1	hypothetical protein	-
L5I26_RS13520 (70907)	70907..71299	+	393	WP_049098530.1	hypothetical protein	-
L5I26_RS13525 (71292)	71292..73358	+	2067	WP_142425810.1	MobP2 family relaxase	-
L5I26_RS13530 (73464)	73464..74021	+	558	WP_053087739.1	hypothetical protein	-
L5I26_RS13535 (74341)	74341..74526	+	186	WP_141435780.1	hypothetical protein	-
L5I26_RS13540 (74564)	74564..75415	+	852	WP_142425809.1	DNA/RNA non-specific endonuclease	-
L5I26_RS13545 (75611)	75611..75871	+	261	WP_071865807.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
L5I26_RS13550 (75858)	75858..76136	+	279	WP_049098536.1	type II toxin-antitoxin system YafQ family toxin	Toxin
L5I26_RS13555 (76171)	76171..76602	+	432	WP_049098537.1	hypothetical protein	-
L5I26_RS13560 (76993)	76993..77205	+	213	WP_049098538.1	hypothetical protein	-
L5I26_RS13565 (77301)	77301..78278	+	978	WP_142425808.1	S41 family peptidase	-
L5I26_RS13570 (78328)	78328..78699	+	372	WP_142425807.1	transposase	-
L5I26_RS13575 (78874)	78874..79212	+	339	WP_049098541.1	hypothetical protein	-
L5I26_RS13580 (79280)	79280..79480	+	201	WP_141441977.1	hypothetical protein	-
L5I26_RS13585 (79550)	79550..80899	+	1350	WP_172497090.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / erm(B)	cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / EF0149 / asa1	1..149744	149744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-54)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11241.23 Da
• Isoelectric Point: 10.1574

>T235233 WP_049098536.1 NZ_CP091907:75858-76136 [Enterococcus faecalis]
MLTIEYTSIFKRDYKRMVKKHYNMKKLEKVVKLLVTNNQDELIRKYKDHALKGNWKGYRELHLDKDWLLIYKIDDKNLIL
TMTRTGSHDELL

Nucleotide

• Length: 279 bp

>T235233 NZ_LR134228:4215097-4215438 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9815.14 Da
• Isoelectric Point: 4.3654

>AT235233 WP_071865807.1 NZ_CP091907:75611-75871 [Enterococcus faecalis]
MTVSRLNIRIDGDLKEQAKEVYKDMGMDLTTAVTIFLKQSVREQRLPFQPSREPIENVIARYEVENGLTTKVDSVADLME
NLNADD

Nucleotide

• Length: 261 bp

>AT235233 NZ_LR134228:c4215102-4215026 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A1L8R1L1