Detailed information of TA system TA023522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2991325..2991922
• Replicon: chromosome
• Accession: NC_011979
• Organism: Geobacter daltonii FRC-32

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B9M1J2
• Locus tag: GEOB_RS13515
• Protein ID: WP_012647802.1
• Coordinates: 2991641..2991922 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GEOB_RS13510
• Protein ID: WP_012647801.1
• Coordinates: 2991325..2991630 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEOB_RS13475	2986745..2987449	+	705	WP_012647794.1	DNA lyase	-
GEOB_RS13480	2987446..2988036	+	591	WP_012647795.1	7-cyano-7-deazaguanine synthase	-
GEOB_RS13485	2988038..2988502	+	465	WP_012647796.1	TIR domain-containing protein	-
GEOB_RS13490	2988502..2989089	+	588	WP_012647797.1	hypothetical protein	-
GEOB_RS13495	2989207..2990058	+	852	WP_012647798.1	hypothetical protein	-
GEOB_RS13500	2990188..2990790	+	603	WP_012647799.1	hypothetical protein	-
GEOB_RS13505	2990919..2991215	+	297	WP_012647800.1	DUF167 domain-containing protein	-
GEOB_RS13510	2991325..2991630	-	306	WP_012647801.1	HigA family addiction module antidote protein	Antitoxin
GEOB_RS13515	2991641..2991922	-	282	WP_012647802.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GEOB_RS13520	2992000..2992830	+	831	WP_012647803.1	hypothetical protein	-
GEOB_RS19970	2992871..2993389	+	519	WP_012647804.1	hypothetical protein	-
GEOB_RS19820	2993395..2993682	+	288	Protein_2688	hypothetical protein	-
GEOB_RS13530	2993898..2994812	-	915	WP_012647806.1	transporter	-
GEOB_RS13535	2994922..2995671	-	750	WP_012647807.1	cytochrome C biogenesis protein CcdA	-
GEOB_RS13540	2995685..2996191	-	507	WP_012647808.1	TlpA family protein disulfide reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10829.34 Da
• Isoelectric Point: 9.9018

>T23522 WP_012647802.1 NC_011979:c2991922-2991641 [Geobacter daltonii FRC-32]
MIKTFACKETEKLFHGRFSGKLPQDVQRIAQRKLKQIHGATTLDFLRVPPGNRLEQLSGSRTGQWSIRINDQWRICFTWQ
DGNATDVEIVDYH

Nucleotide

• Length: 282 bp

>T23522 NC_011979:c2991922-2991641 [Geobacter daltonii FRC-32]
ATGATCAAGACGTTTGCCTGTAAAGAGACTGAAAAGCTTTTTCACGGCCGCTTTTCCGGAAAGCTGCCACAGGATGTGCA
GCGTATCGCCCAACGGAAGCTAAAACAGATTCATGGCGCCACTACCCTGGATTTTCTCCGCGTACCACCAGGCAACAGGC
TTGAGCAACTTTCAGGGAGCCGCACCGGCCAGTGGAGCATCCGAATCAACGACCAATGGCGCATCTGTTTTACCTGGCAA
GACGGAAATGCAACGGACGTCGAAATCGTCGATTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11490.33 Da
• Isoelectric Point: 7.8277

>AT23522 WP_012647801.1 NC_011979:c2991630-2991325 [Geobacter daltonii FRC-32]
MIKRDLPPVHPGEILLEDFLKPLNVTRYRLAKSIGVPQRRIDEICAGKRAITADTALRLARFFGTDAQSWMNLQAQYDLE
CAEITLAERIEQEVKPLRNAA

Nucleotide

• Length: 306 bp

>AT23522 NC_011979:c2991630-2991325 [Geobacter daltonii FRC-32]
ATGATAAAACGCGATCTTCCCCCGGTACATCCTGGAGAAATTCTCCTTGAAGACTTTCTAAAGCCGCTAAACGTTACCCG
CTATAGGCTTGCCAAGTCCATCGGCGTACCCCAACGCCGTATCGATGAAATCTGCGCCGGCAAACGGGCCATCACTGCCG
ACACCGCCTTGCGCCTTGCCCGGTTCTTCGGCACAGACGCCCAAAGCTGGATGAATCTTCAAGCCCAATACGATCTCGAA
TGTGCTGAAATCACTTTGGCTGAGCGAATCGAGCAAGAAGTGAAACCTCTCAGAAATGCAGCTTAG

Structures

Toxin

• AlphaFold DB: B9M1J2