Detailed information of TA system TA235204

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): fst-ratA/Fst(toxin)
• Location: 52435..52676
• Replicon: plasmid p1
• Accession: NZ_CP091905
• Organism: Enterococcus faecalis strain 43-2

Toxin (Protein)

• Gene name: fst
• Uniprot ID: -
• Locus tag: L5I20_RS15150
• Protein ID: WP_002360667.1
• Coordinates: 52435..52545 (+)
• Length: 37 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	52585..52676 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I20_RS15100 (47526)	47526..47693	-	168	Protein_53	peptide-binding protein	-
L5I20_RS15105 (47827)	47827..48087	-	261	Protein_54	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
L5I20_RS15110 (48267)	48267..48947	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
L5I20_RS15115 (48989)	48989..49279	+	291	WP_238461556.1	hypothetical protein	-
L5I20_RS15120 (49273)	49273..49497	+	225	WP_002406593.1	ultraviolet resistance protein UvrA repressor UvrC	-
L5I20_RS15125 (49558)	49558..49767	+	210	WP_010822384.1	hypothetical protein	-
L5I20_RS15130 (49779)	49779..50081	+	303	WP_002406176.1	DUF6440 family protein	-
L5I20_RS15135 (50509)	50509..51837	+	1329	WP_211137039.1	ultraviolet resistance protein UvrA	-
L5I20_RS15140 (51834)	51834..52184	+	351	WP_002400993.1	hypothetical protein	-
L5I20_RS15145 (52141)	52141..52353	+	213	WP_002360669.1	hypothetical protein	-
L5I20_RS15150 (52435)	52435..52545	+	111	WP_002360667.1	type I toxin-antitoxin system Fst family toxin	Toxin
- (52585)	52585..52676	-	92	NuclAT_0	-	Antitoxin
- (52585)	52585..52676	-	92	NuclAT_0	-	Antitoxin
- (52585)	52585..52676	-	92	NuclAT_0	-	Antitoxin
- (52585)	52585..52676	-	92	NuclAT_0	-	Antitoxin
L5I20_RS15155 (52785)	52785..53075	+	291	WP_049084802.1	hypothetical protein	-
L5I20_RS15160 (53179)	53179..53550	-	372	WP_000049959.1	replication-associated protein RepC	-
L5I20_RS15165 (53543)	53543..54388	-	846	WP_000239313.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B)	cylR2 / cylL / cylS / cylM / cylB / cylA / cylI	1..54858	54858
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	30868..48947	18079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 37 a.a.
• Molecular weight: 4117.92 Da
• Isoelectric Point: 4.1672

>T235204 WP_002360667.1 NZ_CP091905:52435-52545 [Enterococcus faecalis]
VLFVKDLMSLVIAPIFVGLVLEMISRVLDEEDDNRK

Nucleotide

• Length: 111 bp

>T235204 NZ_LR134227:2570957-2571064 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 92 bp

>AT235204 NZ_CP091905:c52676-52585 [Enterococcus faecalis]
TAAAAATATGTTATACTAAAGGTGCGAAACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGA
TTGCTTTTTTTT

Structures

Antitoxin