Detailed information of TA system TA235174

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2998326..2998663
• Replicon: chromosome
• Accession: NZ_CP091901
• Organism: Enterococcus faecalis strain 59

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E2Z0W4
• Locus tag: L5I17_RS14575
• Protein ID: WP_002381035.1
• Coordinates: 2998326..2998469 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2998401..2998663 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I17_RS14560	2993551..2994180	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
L5I17_RS14565	2994873..2996489	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-
L5I17_RS14570	2996598..2997890	-	1293	WP_000122610.1	ISL3-like element IS1251 family transposase	-
L5I17_RS14575	2998326..2998469	+	144	WP_002381035.1	putative holin-like toxin	Toxin
-	2998401..2998663	-	263	-	-	Antitoxin
L5I17_RS14580	2998664..3003355	-	4692	WP_010783624.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2996598..2997890	1292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5202.20 Da
• Isoelectric Point: 10.0041

>T235174 WP_002381035.1 NZ_CP091901:2998326-2998469 [Enterococcus faecalis]
MNVSTKIYERRGLLSIAEALALMISFGSFIATLIFGILEAVKENNKK

Nucleotide

• Length: 144 bp

>T235174 NZ_LR134226:860098-860505 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 263 bp

>AT235174 NZ_CP091901:c2998663-2998401 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A2Z6BWH2

Antitoxin