Detailed information of TA system TA235023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 613931..614163
• Replicon: chromosome
• Accession: NZ_CP091889
• Organism: Enterococcus faecalis strain 142-1

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: C7CXQ5
• Locus tag: L5I18_RS03095
• Protein ID: WP_002355568.1
• Coordinates: 613931..614047 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	613958..614163 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I18_RS03085 (609369)	609369..611627	+	2259	WP_002363179.1	DNA helicase PcrA	-
L5I18_RS03090 (611753)	611753..613783	+	2031	WP_010710796.1	NAD-dependent DNA ligase LigA	-
L5I18_RS03095 (613931)	613931..614047	+	117	WP_002355568.1	putative holin-like toxin	Toxin
- (613958)	613958..614163	-	206	NuclAT_3	-	Antitoxin
L5I18_RS03100 (614365)	614365..614670	+	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
L5I18_RS03105 (614670)	614670..616139	+	1470	WP_033593283.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
L5I18_RS03110 (616139)	616139..617569	+	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
L5I18_RS03115 (617588)	617588..618676	+	1089	WP_002355572.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4065.93 Da
• Isoelectric Point: 5.9482

>T235023 WP_002355568.1 NZ_CP091889:613931-614047 [Enterococcus faecalis]
MFLSVEAALGLMIGFATLVVTIIFGILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T235023 NZ_LR134189:c3843890-3843603 [Providencia rustigianii]
ATGAGTATAAAAATAAAAGAATATGACATCGCTGAGCATCTGGCGAGCGAAGAAGAAATCCAATTATATTTGAATGAAAT
ACTTGAAGAAGGCGATCCCGCATTAATACTGTCAGCGTTAGGTGATATTGCTAGAGCGAGAAATATGACTCAGCTGGCTA
AAGATGTTGGGATGAGTCGAGAAGGCTTATATAAAGCGTTGTCTGGTCAGGGAAATCCAACGTTTACAACGATTATGAAA
ATCGTCCATTCTCTTGGATTCAAATTACAGTTTAAAAGGGCGCTGTAA

Antitoxin

Nucleotide

• Length: 206 bp

>AT235023 NZ_CP091889:c614163-613958 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A1B4XLM6

Antitoxin