Detailed information of TA system TA023494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 43617..44208
• Replicon: chromosome
• Accession: NC_011901
• Organism: Thioalkalivibrio sulfidophilus HL-EbGr7

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B8GT88
• Locus tag: TGR7_RS00215
• Protein ID: WP_012636637.1
• Coordinates: 43930..44208 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TGR7_RS00210
• Protein ID: WP_012636636.1
• Coordinates: 43617..43916 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TGR7_RS00195	39215..41149	-	1935	WP_148211529.1	HAMP domain-containing histidine kinase	-
TGR7_RS16910	41305..41463	+	159	WP_012636633.1	lipoprotein	-
TGR7_RS00200	41562..42806	+	1245	WP_012636634.1	diaminopimelate decarboxylase	-
TGR7_RS00205	42929..43600	+	672	WP_012636635.1	class I SAM-dependent methyltransferase	-
TGR7_RS00210	43617..43916	-	300	WP_012636636.1	HigA family addiction module antidote protein	Antitoxin
TGR7_RS00215	43930..44208	-	279	WP_012636637.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TGR7_RS00220	44376..45212	+	837	WP_012636638.1	diaminopimelate epimerase	-
TGR7_RS00225	45209..45895	+	687	WP_012636639.1	DUF484 family protein	-
TGR7_RS00230	46068..46964	+	897	WP_012636640.1	tyrosine recombinase XerC	-
TGR7_RS00235	47044..47991	+	948	WP_041442164.1	AAA family ATPase	-
TGR7_RS16675	47995..48990	+	996	WP_012636642.1	DUF58 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10648.07 Da
• Isoelectric Point: 9.2196

>T23494 WP_012636637.1 NC_011901:c44208-43930 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MIKSFADKDTRALFEGGDPRRFRAFRAQAERKLALLDAATTLEFLRSPPGNRLEALTGDRKGQWSIRINQQWRLCFCWQD
GNAYDVGIVDYH

Nucleotide

• Length: 279 bp

>T23494 NC_011901:c44208-43930 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGATCAAGAGCTTCGCGGATAAGGATACCCGCGCCTTGTTCGAAGGCGGTGACCCACGCCGCTTTCGGGCATTTCGCGC
TCAGGCCGAGCGGAAGCTGGCCCTGCTCGATGCCGCAACGACGCTGGAATTCCTGCGCAGTCCACCGGGCAACCGCCTCG
AGGCCCTGACCGGCGACCGGAAAGGACAATGGAGTATCCGCATCAACCAGCAGTGGCGATTGTGCTTCTGCTGGCAAGAT
GGCAATGCCTATGACGTCGGGATCGTCGACTATCACTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11102.81 Da
• Isoelectric Point: 10.3988

>AT23494 WP_012636636.1 NC_011901:c43916-43617 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MATHRMRPIHPGEILREEYLAPLGMSVNALARALRVPATRLHEIVNERRAVSADTALRLARFFGGDAQSWLNLQSTYELR
LAEAERGDIIKKEVAPRAA

Nucleotide

• Length: 300 bp

>AT23494 NC_011901:c43916-43617 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGGCCACCCACCGCATGCGTCCCATTCATCCAGGGGAGATCCTGCGCGAGGAGTATCTGGCGCCACTTGGCATGAGCGT
GAATGCCCTGGCCCGCGCGTTGCGGGTCCCGGCCACGCGCCTGCATGAAATCGTCAATGAACGTCGTGCAGTGAGCGCGG
ACACCGCCTTGCGTCTCGCCCGTTTCTTCGGCGGGGACGCCCAGTCCTGGCTGAATCTTCAGAGCACCTATGAGCTCCGC
CTGGCCGAGGCCGAGCGCGGCGACATCATCAAGAAAGAGGTCGCGCCCCGCGCGGCGTGA

Structures

Toxin

• AlphaFold DB: B8GT88