Detailed information of TA system TA234913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1629343..1629766
• Replicon: chromosome
• Accession: NZ_CP091875
• Organism: Staphylococcus aureus strain MN8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6B5KJM6
• Locus tag: MAK07_RS07715
• Protein ID: WP_001065110.1
• Coordinates: 1629515..1629766 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E1X568
• Locus tag: MAK07_RS07710
• Protein ID: WP_000028425.1
• Coordinates: 1629343..1629525 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAK07_RS07670 (1624736)	1624736..1625026	-	291	WP_000665205.1	VRR-NUC domain-containing protein	-
MAK07_RS07675 (1625160)	1625160..1625264	+	105	WP_015978074.1	hypothetical protein	-
MAK07_RS07680 (1625367)	1625367..1627814	-	2448	WP_000884879.1	virulence-associated E family protein	-
MAK07_RS07685 (1627867)	1627867..1628067	-	201	WP_000265258.1	DUF1514 family protein	-
MAK07_RS07690 (1628135)	1628135..1628287	-	153	WP_000595237.1	transcriptional activator RinB	-
MAK07_RS07695 (1628280)	1628280..1628516	-	237	WP_000483477.1	hypothetical protein	-
MAK07_RS07700 (1628541)	1628541..1628777	-	237	WP_000195806.1	DUF1381 domain-containing protein	-
MAK07_RS07705 (1628814)	1628814..1629350	-	537	WP_000185703.1	dUTPase	-
MAK07_RS07710 (1629343)	1629343..1629525	-	183	WP_000028425.1	hypothetical protein	Antitoxin
MAK07_RS07715 (1629515)	1629515..1629766	-	252	WP_001065110.1	DUF1024 family protein	Toxin
MAK07_RS07720 (1629781)	1629781..1630023	-	243	WP_000132193.1	SAV1978 family virulence-associated passenger protein	-
MAK07_RS07725 (1630038)	1630038..1630238	-	201	WP_000235324.1	hypothetical protein	-
MAK07_RS07730 (1630241)	1630241..1630498	-	258	WP_000022730.1	DUF3310 domain-containing protein	-
MAK07_RS07735 (1630498)	1630498..1630899	-	402	WP_000113974.1	PVL family protein	-
MAK07_RS07740 (1630899)	1630899..1631084	-	186	WP_001164629.1	DUF3113 family protein	-
MAK07_RS07745 (1631097)	1631097..1633049	-	1953	WP_078056499.1	DNA polymerase	-
MAK07_RS07750 (1633117)	1633117..1633673	-	557	Protein_1526	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ebp / gnd	1582334..1668100	85766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9405.32 Da
• Isoelectric Point: 3.8051

>T234913 WP_001065110.1 NZ_CP091875:c1629766-1629515 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKARAFDEILEGLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T234913 NZ_LR134139:847405-847725 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTA
A

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6781.65 Da
• Isoelectric Point: 4.4421

>AT234913 WP_000028425.1 NZ_CP091875:c1629525-1629343 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDGSIISLIDFVIKPIHLIKEAQEND

Nucleotide

• Length: 183 bp

>AT234913 NZ_LR134139:847193-847402 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A6B5KJM6

Antitoxin

• AlphaFold DB: A0A0E1X568