Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2302191..2302918 Replicon chromosome
Accession NZ_CP091839
Organism Escherichia coli strain SWYHX138.261ft

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L7H22_RS11040 Protein ID WP_000550189.1
Coordinates 2302604..2302918 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L7H22_RS11035 Protein ID WP_000560266.1
Coordinates 2302191..2302607 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H22_RS11025 (2297551) 2297551..2299902 + 2352 WP_000695487.1 alpha-glucosidase -
L7H22_RS11030 (2300128) 2300128..2302146 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L7H22_RS11035 (2302191) 2302191..2302607 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L7H22_RS11040 (2302604) 2302604..2302918 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L7H22_RS11045 (2303199) 2303199..2304335 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L7H22_RS11050 (2304420) 2304420..2304923 + 504 WP_238059220.1 M48 family metallopeptidase -
L7H22_RS11055 (2305000) 2305000..2305692 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L7H22_RS11060 (2305771) 2305771..2306757 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T234869 WP_000550189.1 NZ_CP091839:c2302918-2302604 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T234869 NZ_LR134093:c2159657-2159295 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT234869 WP_000560266.1 NZ_CP091839:c2302607-2302191 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT234869 NZ_LR134093:c2159824-2159654 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References