Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2301527..2302254 Replicon chromosome
Accession NZ_CP091836
Organism Escherichia coli strain SWYHX138.33gt

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L7H17_RS11035 Protein ID WP_000550189.1
Coordinates 2301940..2302254 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L7H17_RS11030 Protein ID WP_000560266.1
Coordinates 2301527..2301943 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H17_RS11020 (2296887) 2296887..2299238 + 2352 WP_000695487.1 alpha-glucosidase -
L7H17_RS11025 (2299464) 2299464..2301482 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L7H17_RS11030 (2301527) 2301527..2301943 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L7H17_RS11035 (2301940) 2301940..2302254 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L7H17_RS11040 (2302535) 2302535..2303671 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L7H17_RS11045 (2303756) 2303756..2304259 + 504 WP_238059220.1 M48 family metallopeptidase -
L7H17_RS11050 (2304336) 2304336..2305028 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L7H17_RS11055 (2305107) 2305107..2306093 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T234847 WP_000550189.1 NZ_CP091836:c2302254-2301940 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T234847 NZ_LR134092:2207033-2207136 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT234847 WP_000560266.1 NZ_CP091836:c2301943-2301527 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT234847 NZ_LR134092:c2207174-2206903 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References