Detailed information of TA system TA234831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 201114..201845
• Replicon: plasmid unnamed
• Accession: NZ_CP091834
• Organism: Shewanella xiamenensis strain HD6452

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K5M76_RS22680
• Protein ID: WP_259474369.1
• Coordinates: 201543..201845 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5M76_RS22675
• Protein ID: WP_259474368.1
• Coordinates: 201114..201533 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5M76_RS22650 (K5M76_22615)	197273..197896	-	624	WP_172589345.1	hypothetical protein	-
K5M76_RS22655 (K5M76_22620)	199253..199984	-	732	WP_259476949.1	endonuclease	-
K5M76_RS22660 (K5M76_22625)	199981..200385	-	405	WP_259476950.1	hypothetical protein	-
K5M76_RS22665 (K5M76_22630)	200401..200571	-	171	WP_259476951.1	hypothetical protein	-
K5M76_RS22670 (K5M76_22635)	200786..201034	-	249	WP_139122718.1	hypothetical protein	-
K5M76_RS22675 (K5M76_22640)	201114..201533	-	420	WP_259474368.1	helix-turn-helix domain-containing protein	Antitoxin
K5M76_RS22680 (K5M76_22645)	201543..201845	-	303	WP_259474369.1	type II toxin-antitoxin system HigB family toxin	Toxin
K5M76_RS22685 (K5M76_22650)	201998..202663	-	666	WP_259474370.1	site-specific integrase	-
K5M76_RS22690 (K5M76_22655)	203263..204051	-	789	WP_014611682.1	hypothetical protein	-
K5M76_RS22695 (K5M76_22660)	204104..204409	-	306	WP_014611681.1	hypothetical protein	-
K5M76_RS22700 (K5M76_22665)	204461..205642	-	1182	WP_259476952.1	toprim domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaNDM-1 / qnrVC6 / blaCMY-10 / mph(A) / blaTEM-1B / aac(3)-IId / msr(E) / mph(E) / tet(C) / aadA6	-	1..256918	256918
	-	flank	IS/Tn	-	-	198550..198651	101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11794.67 Da
• Isoelectric Point: 10.1722

>T234831 WP_259474369.1 NZ_CP091834:c201845-201543 [Shewanella xiamenensis]
MHVISRKPFADAIKKFPNDASAIESTYNVLKRGDFKTPQELRAVFGSLDNFKYKDKWWIIDIGGNNLRMLAFIEFVHNRI
YVKHIVSHSEYDALCKKYRK

Nucleotide

• Length: 303 bp

>T234831 NZ_LR134091:c2099496-2099287 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15282.52 Da
• Isoelectric Point: 4.4049

>AT234831 WP_259474368.1 NZ_CP091834:c201533-201114 [Shewanella xiamenensis]
MLAVAELTKLAGNIMSNVPLLAGISNAAEHQQALELMEELLDDYDANLVVIEALSNVIARYEDESLRFDMFNSRQAELDP
AVATLKVLMDQNGLNTTDFEAEIGKKSMVSQVLAGKKNLTKEHISKLAQRFGVNPAVFF

Nucleotide

• Length: 420 bp

>AT234831 NZ_LR134091:c2099801-2099508 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA