Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3862328..3862925 Replicon chromosome
Accession NZ_CP091813
Organism Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag L7F76_RS19235 Protein ID WP_004142563.1
Coordinates 3862608..3862925 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag L7F76_RS19230 Protein ID WP_004142561.1
Coordinates 3862328..3862615 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7F76_RS19200 (3858422) 3858422..3858670 + 249 WP_002893037.1 DUF1158 domain-containing protein -
L7F76_RS19205 (3858688) 3858688..3859029 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
L7F76_RS19210 (3859060) 3859060..3860174 - 1115 Protein_3765 MBL fold metallo-hydrolase -
L7F76_RS19215 (3860353) 3860353..3860922 + 570 WP_062920870.1 TetR/AcrR family transcriptional regulator -
L7F76_RS19220 (3860922) 3860922..3861290 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
L7F76_RS19225 (3861410) 3861410..3862063 + 654 Protein_3768 oxygen-insensitive NAD(P)H nitroreductase -
L7F76_RS19230 (3862328) 3862328..3862615 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
L7F76_RS19235 (3862608) 3862608..3862925 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L7F76_RS19240 (3863110) 3863110..3864153 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
L7F76_RS19245 (3864820) 3864820..3865686 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
L7F76_RS19250 (3865795) 3865795..3867222 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T234817 WP_004142563.1 NZ_CP091813:c3862925-3862608 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T234817 NZ_LR134089:1006027-1006212 [Staphylococcus saprophyticus]
ATGAGTTTAGATAAAAATCAAAATAATCTACATCGTGATGAATTAGAGTCAGAAAACGCGATGGTTGATGATTTAGAGGA
TGTCGTAGAATTAGGAAAAGAAATGGAACAAATCTCTGAAGAAAATGATGAAGATAAATTAGATAAATCACATGACCCAG
CAGTACGTTCCGATTTAAATGAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT234817 WP_004142561.1 NZ_CP091813:c3862615-3862328 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT234817 NZ_LR134089:1005406-1006005 [Staphylococcus saprophyticus]
ATGGCAATGAACTTTAAAGTTTTAGAAAATGAACAAGTTGTAGCGGAATATGCTGCAGATATCTTAAGAAAACAATTTAA
CAACAATCCAACAACAATTGCTGGTGTACACCTTTCAAGTGACAATGCACCAGTATTGGATGAATTAAAGAAAAATGTTG
ATAAACATGCAGTAGATTTTAGTCAAATTAATATTCTGGATTATGATAACAATAAATCATACTTTGAAGCACTTGGTGTT
CCAGAAGGCCAAATCTTCAATGTTTCATTTGGTGAAGATACGGAATCATTTATTAAAGATAAAATCAAAACTAAAGAAAA
TAAAGGGAAATTAATTACACAAGTACTTTCTATTGATACAAATGGTAAATTAGATGTAAGTGTGAATCAAGGTTTATTCT
CGTCACGTGAAGTTATTTTAATTATTACAGGTAGCAATAAAAAAGAAGTCGTTCATAAACTTTATGAAGAAAATGGTAAA
ACAAGTTTTGAACCTTCAGATTTAAAAGCGCACCGTATGGTTAATGTTATTTTAGATGAAGCGGCTGCTGAAGGTTTACC
AGAAGATGTTAGACATTATTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References