Detailed information of TA system TA234803

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22952..23378
• Replicon: plasmid p3_21223
• Accession: NZ_CP091808
• Organism: Klebsiella quasipneumoniae strain 21223

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L7H25_RS27160
• Protein ID: WP_001372321.1
• Coordinates: 22952..23077 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23154..23378 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7H25_RS27125 (17975)	17975..18202	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
L7H25_RS27130 (18339)	18339..19010	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
L7H25_RS27135 (19204)	19204..19587	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L7H25_RS27140 (19922)	19922..20512	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
L7H25_RS27145 (20809)	20809..21630	-	822	WP_001234445.1	DUF932 domain-containing protein	-
L7H25_RS27150 (21741)	21741..22037	-	297	WP_001272251.1	hypothetical protein	-
L7H25_RS27155 (22337)	22337..22633	+	297	Protein_33	hypothetical protein	-
L7H25_RS27160 (22952)	22952..23077	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L7H25_RS27165 (23019)	23019..23168	-	150	Protein_35	plasmid maintenance protein Mok	-
- (23154)	23154..23378	-	225	NuclAT_0	-	Antitoxin
- (23154)	23154..23378	-	225	NuclAT_0	-	Antitoxin
- (23154)	23154..23378	-	225	NuclAT_0	-	Antitoxin
- (23154)	23154..23378	-	225	NuclAT_0	-	Antitoxin
L7H25_RS27170 (23390)	23390..24109	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
L7H25_RS27175 (24106)	24106..24540	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L7H25_RS27180 (24609)	24609..26632	-	2024	Protein_38	ParB/RepB/Spo0J family partition protein	-
L7H25_RS27185 (26693)	26693..26926	-	234	WP_000006003.1	DUF905 family protein	-
L7H25_RS27190 (26984)	26984..27511	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
L7H25_RS27195 (27813)	27813..28262	+	450	Protein_41	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234803 WP_001372321.1 NZ_CP091808:c23077-22952 [Klebsiella quasipneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234803 NZ_LR134086:c2239243-2239139 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTAATCAAGGCCATAAAAAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT234803 NZ_CP091808:c23378-23154 [Klebsiella quasipneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin