Detailed information of TA system TA023479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 558266..558909
• Replicon: chromosome
• Accession: NC_011852
• Organism: Haemophilus parasuis SH0165

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: HAPS_RS02545
• Protein ID: WP_005714197.1
• Coordinates: 558565..558909 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAPS_RS02540
• Protein ID: WP_012621770.1
• Coordinates: 558266..558562 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAPS_RS02515	553596..553844	-	249	WP_041603119.1	hypothetical protein	-
HAPS_RS02520	553807..555735	-	1929	WP_012621767.1	ATP-dependent zinc metalloprotease FtsH	-
HAPS_RS02525	555809..556435	-	627	WP_010786866.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-
HAPS_RS02530	556678..557466	+	789	WP_012621768.1	SDR family oxidoreductase	-
HAPS_RS02535	557546..558208	+	663	WP_012621769.1	Fic family protein	-
HAPS_RS02540	558266..558562	-	297	WP_012621770.1	helix-turn-helix domain-containing protein	Antitoxin
HAPS_RS02545	558565..558909	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAPS_RS02550	559124..561103	+	1980	WP_012621771.1	exoribonuclease II	-
HAPS_RS02555	561214..562305	+	1092	WP_012621772.1	murein transglycosylase A	-
HAPS_RS02560	562568..563196	+	629	Protein_516	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T23479 WP_005714197.1 NC_011852:c558909-558565 [Glaesserella parasuis SH0165]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T23479 NC_011852:c558909-558565 [Glaesserella parasuis SH0165]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCTCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11224.13 Da
• Isoelectric Point: 10.0887

>AT23479 WP_012621770.1 NC_011852:c558562-558266 [Glaesserella parasuis SH0165]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPNLVPVLQNL

Nucleotide

• Length: 297 bp

>AT23479 NC_011852:c558562-558266 [Glaesserella parasuis SH0165]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAATCTTGTGCAGTCGTGGGAAATTGGCAGACGAACCCCAAATGGCGCTGCACGGAAA
TTGCTTGCGATTATTGAACAACAGCCTAATCTTGTTCCAGTGCTTCAGAATTTGTAG

Structures

Toxin

• AlphaFold DB: U4SMB1