Detailed information of TA system TA023478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 415338..415899
• Replicon: chromosome
• Accession: NC_011852
• Organism: Haemophilus parasuis SH0165

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B8F405
• Locus tag: HAPS_RS01870
• Protein ID: WP_012621703.1
• Coordinates: 415621..415899 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: HAPS_RS01865
• Protein ID: WP_005712616.1
• Coordinates: 415338..415610 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAPS_RS01845	411724..412242	+	519	WP_012621700.1	single-stranded DNA-binding protein	-
HAPS_RS01850	412450..413664	+	1215	WP_012621701.1	ATP-binding protein	-
HAPS_RS01855	413736..414044	-	309	Protein_378	DUF853 family protein	-
HAPS_RS01860	414054..415274	-	1221	WP_012621702.1	ATP-dependent RNA helicase RhlB	-
HAPS_RS01865	415338..415610	-	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
HAPS_RS01870	415621..415899	-	279	WP_012621703.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAPS_RS01875	416048..417058	+	1011	WP_005712612.1	ornithine carbamoyltransferase	-
HAPS_RS01880	417405..418667	+	1263	WP_012621704.1	serine hydroxymethyltransferase	-
HAPS_RS01885	418781..420109	+	1329	WP_012621705.1	exodeoxyribonuclease VII large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10384.74 Da
• Isoelectric Point: 9.6620

>T23478 WP_012621703.1 NC_011852:c415899-415621 [Glaesserella parasuis SH0165]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQDNLAGHWSIKVSGNWRLTFKFQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T23478 NC_011852:c415899-415621 [Glaesserella parasuis SH0165]
ATGATTATTTCATTTAAGCATAAAGGATTGAAATTATTTTTTGAAACAGGATCAACCGCAGGTATTCAAGCAAATCACGC
TAGTAAACTTGCTTTTCAATTAGCCACTCTAAACCAAGCAAAAAGTGCTTTAGATATGAATGCACCTAGCTGGAATTTAC
ACCAATTACAAGATAATTTAGCTGGGCATTGGTCTATCAAGGTAAGTGGAAATTGGCGTTTAACTTTTAAATTTCAAAAT
GGTCACGCTGAAGTCGTGGACTATCAAGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT23478 WP_005712616.1 NC_011852:c415610-415338 [Glaesserella parasuis SH0165]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT23478 NC_011852:c415610-415338 [Glaesserella parasuis SH0165]
ATGAAAATGTACAATCCAGCTCACCCAGGGCTTATTTTAAGAGAATATATTGAAGGATATAATGTCACAGAAGTGGCTAA
ACGTTTAGGTGTCACTCGTGTCACGCTCTCTCGTATTTTAAATGGCAAAGCCGCAATTTCACCTGAAATGGCTCTTAGAT
TAAGCCAATTATTAAAAACTAGCCCAGAATTATGGCTTGGTTTACAATCCCAATACGATCTTTGGCAGCTTGAACAACGC
CCAAGTTTTAATATTCAGCCGTTATTTGCATAG

Structures

Toxin

• AlphaFold DB: B8F405