Detailed information of TA system TA234762

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83116..83542
• Replicon: plasmid pB349aT_1
• Accession: NZ_CP091774
• Organism: Escherichia coli strain SWYH.B349aT

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: L6V99_RS23960
• Protein ID: WP_001372321.1
• Coordinates: 83116..83241 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83318..83542 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6V99_RS23920 (78490)	78490..79179	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
L6V99_RS23925 (79366)	79366..79749	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
L6V99_RS23930 (80070)	80070..80672	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
L6V99_RS23935 (80969)	80969..81790	-	822	WP_237878124.1	DUF932 domain-containing protein	-
L6V99_RS23940 (81908)	81908..82195	-	288	WP_000107535.1	hypothetical protein	-
L6V99_RS23945 (82220)	82220..82426	-	207	WP_000547968.1	hypothetical protein	-
L6V99_RS23950 (82496)	82496..82668	+	173	Protein_99	hypothetical protein	-
L6V99_RS23955 (82666)	82666..82896	-	231	WP_071586998.1	hypothetical protein	-
L6V99_RS23960 (83116)	83116..83241	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
L6V99_RS23965 (83183)	83183..83332	-	150	Protein_102	plasmid maintenance protein Mok	-
- (83318)	83318..83542	-	225	NuclAT_0	-	Antitoxin
- (83318)	83318..83542	-	225	NuclAT_0	-	Antitoxin
- (83318)	83318..83542	-	225	NuclAT_0	-	Antitoxin
- (83318)	83318..83542	-	225	NuclAT_0	-	Antitoxin
L6V99_RS23970 (83354)	83354..83542	+	189	WP_001299721.1	hypothetical protein	-
L6V99_RS23975 (83511)	83511..84273	-	763	Protein_104	plasmid SOS inhibition protein A	-
L6V99_RS23980 (84270)	84270..84704	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
L6V99_RS23985 (84759)	84759..84956	-	198	Protein_106	hypothetical protein	-
L6V99_RS23990 (84984)	84984..85217	-	234	WP_000005987.1	DUF905 family protein	-
L6V99_RS23995 (85285)	85285..85824	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
L6V99_RS24000 (85920)	85920..87133	+	1214	WP_139371347.1	IS3 family transposase	-
L6V99_RS24005 (87136)	87136..87369	-	234	WP_185681501.1	single-stranded DNA-binding protein	-
L6V99_RS24010 (87439)	87439..87519	+	81	Protein_111	hypothetical protein	-
L6V99_RS24015 (87702)	87702..87871	-	170	Protein_112	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(B) / catA1 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..109860	109860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T234762 WP_001372321.1 NZ_CP091774:c83241-83116 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T234762 NZ_LR134080:3872661-3872939 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT234762 NZ_CP091774:c83542-83318 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin